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Species: SARS-CoV-2, 501V2 (B.1.351)
Genetic Insert: SARS-CoV-2 501V2 spike D18
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34016740
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.16.430500v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170449 Copy
Species: SARS-CoV-1
Genetic Insert: SARS-CoV-1 spike D28
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32540903
Proper citation: RRID:Addgene_170447 Copy
Species: Homo sapiens
Genetic Insert: RORG isoform 2
Vector Backbone Description: Backbone Marker:LifeSensor; Backbone Size:5476; Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34547329
Proper citation: RRID:Addgene_170329 Copy
Vector Backbone Description: Backbone Size:12778; Vector Backbone:pAC; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33782117
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/
Proper citation: RRID:Addgene_170515 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Marker:Feng Zhang lab (Addgene plasmid #52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Plasmid based on lentiGuide Puro (addgene plasmid #52963).
Please visit https://www.biorxiv.org/content/10.1101/2021.03.02.433398v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170510 Copy
Species: Mus musculus
Genetic Insert: Caveolin1
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:leishmania cell free expression clone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904409
Proper citation: RRID:Addgene_170526 Copy
Species: Mus musculus
Genetic Insert: Cavin4
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904409
Proper citation: RRID:Addgene_170523 Copy
Species: Mus musculus
Genetic Insert: Cavin4
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904409
Proper citation: RRID:Addgene_170524 Copy
Species: Mus musculus
Genetic Insert: Caveolin1
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33904409
Proper citation: RRID:Addgene_170527 Copy
Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORF1 amplified from pBand 3-5 (full length cDNA clone) ligated into BamHI-StuI cut CS2P+.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Proper citation: RRID:Addgene_17048 Copy
Species: Mus musculus
Genetic Insert: PLIN2
Vector Backbone Description: Backbone Size:9594; Vector Backbone:pLenti CMV/TO Puro DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29275994
Proper citation: RRID:Addgene_170573 Copy
Vector Backbone Description: Backbone Size:5409; Vector Backbone:pJCX4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34478714
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438513v2 for BioRxiv preprint
Proper citation: RRID:Addgene_170756 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34152213
Proper citation: RRID:Addgene_170636 Copy
Species: Homo sapiens
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Addgene (Plasmid #14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34102054
Comments: Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318.
Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/
Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318
Proper citation: RRID:Addgene_170774 Copy
Species: Homo sapiens
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Addgene Plasmid #19319; Backbone Size:8083; Vector Backbone:pLJM1-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34102054
Comments: Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318.
Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/
Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318
Proper citation: RRID:Addgene_170775 Copy
Species: Homo sapiens
Genetic Insert: PCNA Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_170818 Copy
Species: Drosophila melanogaster
Genetic Insert: GFP-Msp300KASH
Vector Backbone Description: Backbone Size:9360; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34022041
Comments: Plasmid derived from GFP-Msp300KASH fusion provided by Janice Fischer and described in:
Yu, J. et al. (2006) The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Developmental biology 289, 336-345, doi:10.1016/j.ydbio.2005.10.027
Plasmid first described in:
Ma, J. and Weake, V.M. (2014). Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis. Journal of Visualized Experiments 85. PMID:24686501.
Proper citation: RRID:Addgene_170806 Copy
Species: firefly
Genetic Insert: luc
Vector Backbone Description: Backbone Marker:from Han lab; Backbone Size:8800; Vector Backbone:pBOBI_cs2.0; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32540903
Proper citation: RRID:Addgene_170674 Copy
Species: Homo sapiens
Genetic Insert: ERGIC-53
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4026; Vector Backbone:pDendra2-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051356
Comments: Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory.
Proper citation: RRID:Addgene_170717 Copy
Species: Homo sapiens
Genetic Insert: STAT5A
Vector Backbone Description: Vector Backbone:pTRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34263728
Comments: A constitutively active form of STAT5A was cloned into the lentiviral expression vector.
Proper citation: RRID:Addgene_170662 Copy
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