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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pcDNA3.3_CoV2_501V2 Resource Report Resource Website 10+ mentions |
RRID:Addgene_170449 | SARS-CoV-2 501V2 spike D18 | SARS-CoV-2, 501V2 (B.1.351) | Ampicillin | PMID:34016740 | Please visit https://www.biorxiv.org/content/10.1101/2021.02.16.430500v1 for bioRxiv preprint. | Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V; 18aa deletion in c-terminal tail | 2026-08-15 01:10:08 | 13 |
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pcDNA3.3_CoV1_D28 Resource Report Resource Website 10+ mentions |
RRID:Addgene_170447 | SARS-CoV-1 spike D28 | SARS-CoV-1 | Ampicillin | PMID:32540903 | Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | last 28aa deletion in c-terminal tail | 2026-08-15 01:10:08 | 11 | |
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pESUMO-hRORg2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170329 | RORG isoform 2 | Homo sapiens | Ampicillin | PMID:34547329 | Backbone Marker:LifeSensor; Backbone Size:5476; Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | codon optimized | 2026-08-15 01:10:07 | 1 | |
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pAC-CR7T-gRNA2.1-nlsBFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_170515 | Ampicillin | PMID:33782117 | For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/ | Backbone Size:12778; Vector Backbone:pAC; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:08 | 1 | |||
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LentiGuide Cherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_170510 | S. pyogenes sgRNA cassette | Synthetic | Ampicillin | Plasmid based on lentiGuide Puro (addgene plasmid #52963). Please visit https://www.biorxiv.org/content/10.1101/2021.03.02.433398v1 for bioRxiv preprint. | Backbone Marker:Feng Zhang lab (Addgene plasmid #52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:08 | 2 | ||
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SITS-MmCav1-APEX2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170526 | Caveolin1 | Mus musculus | Ampicillin | PMID:33904409 | Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:leishmania cell free expression clone; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:08 | 1 | ||
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LifeAct-EGFP-APEX2 in pCSDEST2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170523 | Cavin4 | Mus musculus | Ampicillin | PMID:33904409 | Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:08 | 1 | ||
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MmCavin4-APEX2-P2A-mKate2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170524 | Cavin4 | Mus musculus | Ampicillin | PMID:33904409 | Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 1 | ||
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pME-MmCav1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170527 | Caveolin1 | Mus musculus | Kanamycin | PMID:33904409 | Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:09 | 1 | ||
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ZE14 pCS2P+ wnt8 ORF1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_17048 | wnt8 | Danio rerio | Ampicillin | ORF1 amplified from pBand 3-5 (full length cDNA clone) ligated into BamHI-StuI cut CS2P+. For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. | Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 2 | ||
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PLIN2-V5-APEX2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170573 | PLIN2 | Mus musculus | Ampicillin | PMID:29275994 | Backbone Size:9594; Vector Backbone:pLenti CMV/TO Puro DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 2 | ||
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pJCX4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170756 | Kanamycin | PMID:34478714 | Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438513v2 for BioRxiv preprint | Backbone Size:5409; Vector Backbone:pJCX4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:10 | 1 | |||
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pTetQCas-8+IS186 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170636 | VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array) | Vibrio cholera | Streptomycin | PMID:34152213 | Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2026-08-15 01:10:09 | 1 | ||
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pFUGW-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170774 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene (Plasmid #14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:11 | 5 |
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pLJM1-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170775 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene Plasmid #19319; Backbone Size:8083; Vector Backbone:pLJM1-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:10 | 4 |
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AICSDP-116:PCNA-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_170818 | PCNA Homology Arms with mEGFP-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-11 specific homozygous SNP(s) | 2026-08-15 01:10:11 | 1 | |
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pUASTattB-GFP-Msp300KASH Resource Report Resource Website 1+ mentions |
RRID:Addgene_170806 | GFP-Msp300KASH | Drosophila melanogaster | Ampicillin | PMID:34022041 | Plasmid derived from GFP-Msp300KASH fusion provided by Janice Fischer and described in: Yu, J. et al. (2006) The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Developmental biology 289, 336-345, doi:10.1016/j.ydbio.2005.10.027 Plasmid first described in: Ma, J. and Weake, V.M. (2014). Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis. Journal of Visualized Experiments 85. PMID:24686501. | Backbone Size:9360; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 1 | |
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pBOBI-FLuc Resource Report Resource Website 10+ mentions |
RRID:Addgene_170674 | luc | firefly | Ampicillin | PMID:32540903 | Backbone Marker:from Han lab; Backbone Size:8800; Vector Backbone:pBOBI_cs2.0; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 12 | ||
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pmEmerald-ERGIC-53 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170717 | ERGIC-53 | Homo sapiens | Kanamycin | PMID:35051356 | Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory. | Backbone Marker:Clontech; Backbone Size:4026; Vector Backbone:pDendra2-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:10 | 3 | |
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pBR-Lent-STAT5A-CA Resource Report Resource Website 1+ mentions |
RRID:Addgene_170662 | STAT5A | Homo sapiens | Ampicillin | PMID:34263728 | A constitutively active form of STAT5A was cloned into the lentiviral expression vector. | Vector Backbone:pTRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | H298R and S710F | 2026-08-15 01:10:10 | 1 |
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