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Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0040 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109086 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pDSB_0011; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0056 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109087 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0057 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109088 Copy
Species: Synthetic
Genetic Insert: mTFP1
Vector Backbone Description: Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0032 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109080 Copy
Species: Synthetic
Genetic Insert: mito-gTEMP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28212432
Proper citation: RRID:Addgene_109117 Copy
Species: Synthetic
Genetic Insert: nuc-gTEMP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28212432
Proper citation: RRID:Addgene_109118 Copy
Species: Synthetic
Genetic Insert: PTSG_00306 geranyl-geranylation signal
Vector Backbone Description: Vector Backbone:pDSB_0057; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0104 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109096 Copy
Species: Synthetic
Genetic Insert: mTFP1_3'UTR Act
Vector Backbone Description: Vector Backbone:pDSB_0050; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0093 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109097 Copy
Species: Synthetic
Genetic Insert: mCherry_SV40 NLS
Vector Backbone Description: Vector Backbone:pDSB_0057; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0059 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109091 Copy
Species: Synthetic
Genetic Insert: Full-length engineered CD64, derived from human CD64
Vector Backbone Description: Backbone Marker:backbone derived from pACEBac1, Geneva Biotech; Vector Backbone:pGS_C_Strep, Addgene no. 101652; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29143175
Proper citation: RRID:Addgene_109191 Copy
Species: Synthetic
Genetic Insert: attB-Flag-DAS+4
Vector Backbone Description: Backbone Size:3139; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating Flag-DAS4+ fusions to mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109284 Copy
Species: Synthetic
Genetic Insert: Ppsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_109260 Copy
Species: Synthetic
Genetic Insert: Ppsp_pIII(P29*, P83*, T177*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_109263 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Size:3082; Vector Backbone:pKM464; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating deletion of mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109301 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109241 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109235 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109236 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109237 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109251 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109253 Copy
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