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Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11106749
Comments: Addgene sequencing results show 2 mismatches with author's sequence and NCBI sequence for ATF4.
Mouse ATF4 5' UTR and AUG fused to Luciferase driven by the TK promoter in a pGL3 backbone
TK.mATF4.UTR.Luc.pGL3 (5202nt, predicted). TK promoter: 58..184; mATF4 5'UTR and AUG: 185..470; luciferase coding sequence: 472..2121; poly A signals: 2156..2377
Proper citation: RRID:Addgene_21850 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Proper citation: RRID:Addgene_21865 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)
Proper citation: RRID:Addgene_21860 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Proper citation: RRID:Addgene_21864 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15479734
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)
Proper citation: RRID:Addgene_21863 Copy
Species: Mus musculus
Genetic Insert: Pten
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327259
Proper citation: RRID:Addgene_21957 Copy
Species: Mus musculus
Genetic Insert: Pten
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327259
Proper citation: RRID:Addgene_21952 Copy
Species: Mus musculus
Genetic Insert: Pten
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327259
Proper citation: RRID:Addgene_21960 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:1547942
Comments: Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
Proper citation: RRID:Addgene_21899 Copy
Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4310; Vector Backbone:pGL3 and pGL2 modified; Vector Types:Non-viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086
Comments: This reporter gene closely tracks the expression of endogenous CHOP.
Proper citation: RRID:Addgene_21898 Copy
Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Size:4767; Vector Backbone:pcDNA1.1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8650547
Comments: Addgene sequencing results show 6bp insertion in the sequencing adding 2 Gly residues immediately after the Myc tag.
Mammalian expression plasmid encoding wildtype mouse CHOP fused to DNA-binding domain of yeast Gal4.
Proper citation: RRID:Addgene_21914 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: From depositing lab:
"9E10 (Myc-epitope) tagged mouse CHOP coding region only. The insert was joined to the epitope tag using patch-PCR and incorporating unique BamH1 and Xho1 sites for ligation into pcDNA1. Stocks are provided as MC1060 (or MC1061, we are not sure) host strain transformed with the plasmid. Note that pCDNA1 plasmids must be propagated on combined amp and tet selection. I would recommedn that you consider transferring the insert into a better host strain such as pcDNA3"
Proper citation: RRID:Addgene_21913 Copy
Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Addgene sequencing results show an additional mutation in the coding region, causing L850P substitution in the amino acid sequence.
Mouse IRE1b, K536A, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3. The K->A mutation introduces a second MSc1 site into the plasmid at position 2578.
Proper citation: RRID:Addgene_21881 Copy
Species: Mus musculus
Genetic Insert: Oct4i
Vector Backbone Description: Backbone Size:7558; Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19587682
Comments: Oct4 RNAi sequence 5'-GAACCTGGCTAAGCTTCCA
Proper citation: RRID:Addgene_21906 Copy
Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Size:4767; Vector Backbone:pcDNA1.1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: 9E10 (Myc-epitope) tagged mouse CHOP coding region only, S78A and S81A double mutant. The S78A and S81A mutation introduces an additional HhaI site not present in the wildtype insert. Note that there is considerable uncertainity regarding this plasmid.
Proper citation: RRID:Addgene_21902 Copy
Species: Mus musculus
Genetic Insert: FGF2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Comments: Full length coding region, PCR clone. The insert can be cut out with EcoRI and XbaI.
Proper citation: RRID:Addgene_22084 Copy
Species: Mus musculus
Genetic Insert: FGF3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Comments: cDNA with longest 5' end; poly A tail lost because cut with EcoRI.
Proper citation: RRID:Addgene_22086 Copy
Species: Mus musculus
Genetic Insert: Sprouty 2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10498682
Comments: mSprouty cDNA clone was isolated from a mouse E11 cDNA library (Clonetech). The entire coding region as well as 5' and 3' UTR sequences are included.
For antisense probe, linearize the plasmid with SacII and use T3 Polymerase. The transcript should be approximately 1.5kb.
Proper citation: RRID:Addgene_22097 Copy
Species: Mus musculus
Genetic Insert: ATF4/CHOP11
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: record is incomplete on this issue but the preponderance of the evidence suggests that this was the immunogen used to produce the antiserum referred to as "CHOP 11 FL
Proper citation: RRID:Addgene_22130 Copy
Species: Mus musculus
Genetic Insert: GBX2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Comments: The insert contains the full coding sequence and has been used to make RNA probes.
For antisense probe linearize with HindIII and use T7 Polymerase. For sense probe linearize with ApaI or KpnI and transcribe with T3 RNA polymerase.
Proper citation: RRID:Addgene_22092 Copy
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