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Species: Rattus norvegicus
Genetic Insert: N-methyl D-aspartate receptor 2B
Vector Backbone Description: Backbone Size:4716; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11897109
Comments: Addgene NGS identified a few sequence discrepancies relative to Genbank ID: M91562, but the plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_17925 Copy
Species: Rattus norvegicus
Genetic Insert: glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11897109
Proper citation: RRID:Addgene_17926 Copy
Species: Homo sapiens
Genetic Insert: gRNA against TRAC
Vector Backbone Description: Vector Backbone:pXPR_001 (lentiCRISPRv1, Addgene #49535); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_164993 Copy
Species: Homo sapiens
Genetic Insert: TRAV
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_164999 Copy
Species: Homo sapiens
Genetic Insert: Rheb
Vector Backbone Description: Backbone Marker:Available at Addgene (#19319); Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157014
Proper citation: RRID:Addgene_165031 Copy
Species: Homo sapiens
Genetic Insert: BACE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22553349
Proper citation: RRID:Addgene_165032 Copy
Species: Homo sapiens
Genetic Insert: APC
Vector Backbone Description: Backbone Marker:Vogelstein lab (Addgene plasmid# 16440); Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Proper citation: RRID:Addgene_16507 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9660945
Comments: Two copies of the Smad binding site (taaGTCTAGACggcaGTCTAGACgtac) are cloned into pGL3.
Proper citation: RRID:Addgene_16500 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Vector Backbone:pMaster; Vector Types:Mammalian Expression, Episomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088009
Comments: Please note that Addgene's NGS found that the bGH poly(A) signal between one of the oris and Lin28 is absent as compared to the annotated sequence provided by the depositor.
Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.
Proper citation: RRID:Addgene_165081 Copy
Species: Ruminoccocus flavefaciens XPD3002
Genetic Insert: RfxCas13d
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Proper citation: RRID:Addgene_165077 Copy
Species: Other
Genetic Insert: Scaffold gRNA
Vector Backbone Description: Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Comments: Please note that viral vectors are prone to recombination. Addgene recommends testing 2-4 individual colonies to ensure the full plasmid is intact.
Proper citation: RRID:Addgene_165078 Copy
Species: Ruminoccocus flavefaciens XPD3002
Genetic Insert: RfxCas13d
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Comments: Please note that the fluorophore is sfGFP. Previous versions of SnapGene may label it as mCFP.
Proper citation: RRID:Addgene_165076 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Comments: SBE-luc was made by annealing the oligonucleotides 5′-CGGAGTATGTCTAGACTGACAATG-3′ and 5′-CTAGCATTGTCAGTCTAGACATACTCCGAGCT-3′ and cloning them into the Sac1 and Nhe1 sites.
Proper citation: RRID:Addgene_16527 Copy
Species: Homo sapiens
Genetic Insert: FAST-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Comments: To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.
Proper citation: RRID:Addgene_16521 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Comments: HCT116 has a 3-bp deletion that removes one amino acid (Ser45).
Proper citation: RRID:Addgene_16520 Copy
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10555149
Proper citation: RRID:Addgene_16539 Copy
Genetic Insert: 4xACO
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10555149
Comments: Contains 4 copies of the prototypic PPAR-responsive element ACO (5'-AGGACAAAGGTCA-3'). The prototypic PPAR response element ACO from the acyl-CoA oxidase gene promotor contains two copies of the core binding sequence AGGTCA separated by one base pair.
Proper citation: RRID:Addgene_16533 Copy
Species: SARS-COV-2
Genetic Insert: Nsp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165108 Copy
Species: Homo sapiens
Genetic Insert: p53 binding site
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4900; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9659898
Comments: This reporter contains 3 copies of the wt p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGCAT TAGCCCAGAC ATGTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA CATGTCTGGG CTAATGCTAC AGGGTAC-3' for BDS-2.
Proper citation: RRID:Addgene_16515 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.
Proper citation: RRID:Addgene_16514 Copy
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