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Species: Mus musculus
Genetic Insert: Merlin I
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pXJ40-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16247032
Proper citation: RRID:Addgene_19699 Copy
Species: Mus musculus
Genetic Insert: IkB kinase alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5044; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9520401
Proper citation: RRID:Addgene_19739 Copy
Species: Mus musculus
Genetic Insert: IkB kinase alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5044; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9520401
Comments: Splice variant should function the same as pCR-HA-IKKalpha
Proper citation: RRID:Addgene_19738 Copy
Species: Mus musculus
Genetic Insert: oligo targeting p53 and PTEN
Vector Backbone Description: Backbone Size:7994; Vector Backbone:MSCV FLIPi; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19746 Copy
Species: Mus musculus
Genetic Insert: oligo targeting p53
Vector Backbone Description: Backbone Size:7994; Vector Backbone:MSCV FLIPi; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19748 Copy
Species: Mus musculus
Genetic Insert: oligo targeting PTEN
Vector Backbone Description: Backbone Size:7994; Vector Backbone:MSCV FLIPi; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19749 Copy
Species: Mus musculus
Genetic Insert: mouse Brn2
Vector Backbone Description: Backbone Size:4801; Vector Backbone:CAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18667607
Proper citation: RRID:Addgene_19711 Copy
Species: Mus musculus
Genetic Insert: Merlin I
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pXJ40-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16247032
Proper citation: RRID:Addgene_19701 Copy
Species: Mus musculus
Genetic Insert: RASSF1C
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11857081
Proper citation: RRID:Addgene_1977 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Size:9941; Vector Backbone:FUdeltaGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371448
Comments: From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Proper citation: RRID:Addgene_19764 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Backbone Size:9941; Vector Backbone:FUdeltaGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371448
Comments: From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Note that the region immediately upstream of the insert in the author's sequence is not 100% accurate. See Addgene's quality control sequence for this region.
Proper citation: RRID:Addgene_19766 Copy
Species: Mus musculus
Genetic Insert: Sox2
Vector Backbone Description: Backbone Size:9941; Vector Backbone:FUdeltaGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371448
Comments: From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Proper citation: RRID:Addgene_19765 Copy
Species: Mus musculus
Genetic Insert: RASSF1A
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11857081
Proper citation: RRID:Addgene_1980 Copy
Species: Mus musculus
Genetic Insert: Talin 1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110565 Copy
Species: Mus musculus
Genetic Insert: HDAC1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15060137
Comments: Addgene sequencing data finds T189A and D332G substitutions. These are not known to affect function.
Proper citation: RRID:Addgene_11055 Copy
Species: Mus musculus
Genetic Insert: HDAC1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15060137
Proper citation: RRID:Addgene_11054 Copy
Species: Mus musculus
Genetic Insert: HDAC1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCIG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15060137
Comments: pCIG drives simultaneous expression of GFP and inserted genes by virtue of an internal ribosome entry site.
Proper citation: RRID:Addgene_11053 Copy
Species: Mus musculus
Genetic Insert: Grg1-S
Vector Backbone Description: Backbone Marker:Roche Molecular Biochemicals; Backbone Size:5400; Vector Backbone:pMH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12359720
Proper citation: RRID:Addgene_11064 Copy
Species: Mus musculus
Genetic Insert: Grg1-L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12359720
Comments: Mouse orthologue of human TLE1 gene.
Proper citation: RRID:Addgene_11067 Copy
Species: Mus musculus
Genetic Insert: SLPI
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15315966
Proper citation: RRID:Addgene_11069 Copy
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