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Species: Saccharomyces cerevisiae
Genetic Insert: XI2_DW
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198668 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: X4_UP
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198671 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YGL055W
Vector Backbone Description: Backbone Size:6211; Vector Backbone:Yc33 (CEN); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33658354
Proper citation: RRID:Addgene_160653 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scp20_1-17
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148717 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scp20_1-49
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148718 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scp20_1-49
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148719 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScEap1p_101-150
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148711 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScEap1p_107-122
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148712 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4G_408-485
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4G, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P39935) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148713 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4G_448-500
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4G, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P39935) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4E_25-213-KKKKtoAAAA
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4E, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P07260) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148729 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SceIF4E_25-213-I77AI81A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEIF4E, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P07260) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148727 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScEap1p_123-150
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148814 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18644379
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). The protein contains a carboxyl-terminal histidine tag for purification. The expressed protein contains the K122I mutation that results in DNA nicking activity. An appended methionine-histidine sequence is present at the amino terminus.
Proper citation: RRID:Addgene_195614 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin - Leucine - 4FLAG N-terminal tagging
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_193317 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin - Leucine - 4MYC N-terminal tagging
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_193318 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18644379
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The protein contains a carboxyl-terminal histidine tag for purification. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur).
Proper citation: RRID:Addgene_195613 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EMC1
Vector Backbone Description: Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184763 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABF2
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184768 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: IRE1
Vector Backbone Description: Vector Backbone:UG6-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184766 Copy
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