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http://www.addgene.org/210232

Vector Backbone Description: Backbone Marker:Host-Microbe Interactions lab; Vector Backbone:pUdO3c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38235654

Proper citation: RRID:Addgene_210232 Copy   

•   Source: Addgene

http://www.addgene.org/226378

Species: Cellulomonas fimi
Genetic Insert: csgA and csgG,E,F genes of curli operon
Vector Backbone Description: Backbone Size:6211; Vector Backbone:pZa; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39137519

Proper citation: RRID:Addgene_226378 Copy   

•   Source: Addgene

http://www.addgene.org/225538

Species: Magnetospirillum magneticum AMB-1 strain
Genetic Insert: Iron binding protein
Vector Backbone Description: Backbone Size:5289; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35567331

Proper citation: RRID:Addgene_225538 Copy   

•   Source: Addgene

http://www.addgene.org/233589

Species: Synthetic
Genetic Insert: YFV-17D lacking C, prM, E but with mScarlet
Vector Backbone Description: Backbone Size:8888; Vector Backbone:PCCI; Vector Types:Transneural tracing; Bacterial Resistance:Chloramphenicol
Comments: Please view the attached protocol for growing plasmid in the lab. If protocol is not followed, it is likely DNA preps will contain a significant number of DNA impurities.

Proper citation: RRID:Addgene_233589 Copy   

•   Source: Addgene

http://www.addgene.org/232322

Species: Discosoma sp.
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pZP06B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: This plasmid contains mutations in sacB, however, it has been verified to function for counterselection when sucrose is present.

Proper citation: RRID:Addgene_232322 Copy   

•   Source: Addgene

http://www.addgene.org/214104

Species: Homo sapiens
Genetic Insert: MET 5'UTR
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39774381

Proper citation: RRID:Addgene_214104 Copy   

•   Source: Addgene

http://www.addgene.org/214103

Species: Homo sapiens
Genetic Insert: MET 5'UTR
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39774381

Proper citation: RRID:Addgene_214103 Copy   

•   Source: Addgene

http://www.addgene.org/228619

Species: Synthetic
Genetic Insert: LSSmOrange
Vector Backbone Description: Backbone Marker:Birtwistle lab; Vector Backbone:pMulti; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40326708
Comments: Please visit https://doi.org/10.1101/2024.10.23.619855 for bioRxiv preprint.

Proper citation: RRID:Addgene_228619 Copy   

•   Source: Addgene

http://www.addgene.org/214105

Species: Homo sapiens
Genetic Insert: MET 5'UTR uORF2 mutated
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39774381

Proper citation: RRID:Addgene_214105 Copy   

•   Source: Addgene

http://www.addgene.org/214106

Genetic Insert: MET 5'UTR uORF1 and 2 mutated
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39774381

Proper citation: RRID:Addgene_214106 Copy   

•   Source: Addgene

http://www.addgene.org/225428

Species: E. coli
Genetic Insert: mCherry, Cas9, SgRNA
Vector Backbone Description: Backbone Size:3150; Vector Backbone:pSIP401 (spp-based expression) derivative, lacks spp-genes for induction; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39817779

Proper citation: RRID:Addgene_225428 Copy   

•   Source: Addgene

http://www.addgene.org/167227

Species: Other
Genetic Insert: J23119-AKSIRV
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol

Proper citation: RRID:Addgene_167227 Copy   

•   Source: Addgene

http://www.addgene.org/225082

Species: Chryseobacterium sp. JM1
Genetic Insert: ChrA-C63S/C66S
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37252350

Proper citation: RRID:Addgene_225082 Copy   

•   Source: Addgene

http://www.addgene.org/220280

Vector Backbone Description: Backbone Size:5256; Vector Backbone:pSpy0C4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white. Resource Information: The MCS featuring the Plac_mrfp cassette and the chloramphenicol resistance cassette were taken from pSpy1C. The pUC origin for replication in E. coli was taken from pUC19-mKate2∼ssrA. Up- and downstream homologous regions of sagB were amplified from the S. pyogenes SF370 genome. Linearise the plasmid with the restriction enzyme Alw44I before transformation in S. pyogenes to favour double crossover recombination of the plasmid into the sagB locus. Positive transformants will show loss of hemolysis on TSA blood agar plates. Please download the file linked below to obtain a well annotated map of the plasmid.

Proper citation: RRID:Addgene_220280 Copy   

•   Source: Addgene

http://www.addgene.org/218513

Species: Photinus pyralis
Genetic Insert: ffluc
Vector Backbone Description: Vector Backbone:pSpy1C; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: This high-copy replicative shuttle plasmid features an erythromycin-inducible riboswitch driving expression of the Firefly luciferase in the presence of erythromycin. Luminescence can be detected by adding luciferin to the culture. See associated publication for more details. Resource Information: The erythromycin-inducible riboswitch was taken from pLZ12Km2-TA:PgyrA(Sag)-ermBL-ermB':A2I:ffluc-tt3, which was used in the following publication: Dynamics of diversified A-to-I editing in Streptococcus pyogenes is governed by changes in mRNA stability Thomas F. Wulff, Karin Hahnke, Anne-Laure Lécrivain, Katja Schmidt, Rina Ahmed-Begrich, Knut Finstermeier, Emmanuelle Charpentier bioRxiv 2023.09.19.555891; doi: https://doi.org/10.1101/2023.09.19.555891 The ffluc reporter gene was taken from the original pLZ12Km2-P23R:TA:ffluc plasmid (Plasmid #88900). Please download the detailed plasmid map using the file linked below

Proper citation: RRID:Addgene_218513 Copy   

•   Source: Addgene

http://www.addgene.org/220248

Vector Backbone Description: Backbone Size:4917; Vector Backbone:pSpy1C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white. Resource Information: We took the rolling circle replicon pSH71 and the chloramphenicol resistance cassette from pIB166 (Plasmid #90189). The the Plac_mrfp cassette for the MCS is derived from pBS3Clux (Plasmid #55172). Please download the file linked below to obtain a well annotated map of the plasmid.

Proper citation: RRID:Addgene_220248 Copy   

•   Source: Addgene

http://www.addgene.org/194228

Species: Synthetic
Genetic Insert: LCB3_v1.2
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.

Proper citation: RRID:Addgene_194228 Copy   

•   Source: Addgene

http://www.addgene.org/194225

Species: Homo sapiens
Genetic Insert: EF1a Promoter
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.

Proper citation: RRID:Addgene_194225 Copy   

•   Source: Addgene

http://www.addgene.org/194227

Species: Synthetic
Genetic Insert: LCB1_v1.3
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.

Proper citation: RRID:Addgene_194227 Copy   

•   Source: Addgene

http://www.addgene.org/225155

Vector Backbone Description: Backbone Size:2340; Vector Backbone:pSU18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39244484
Comments: Mid copy plasmid (p15a ori) with pUC18 MCS with FLAG epitope integrated at SmaI site, confers chloramphenicol resistance

Proper citation: RRID:Addgene_225155 Copy   

•   Source: Addgene