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Species: Synthetic
Genetic Insert: CaaX domain from Hras
Vector Backbone Description: Vector Backbone:pDONR p2R-p3; Vector Types:Zebrafish plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109539 Copy
Species: Synthetic
Genetic Insert: mCherry T2A GFP
Vector Backbone Description: Backbone Size:9269; Vector Backbone:HIV-1 NL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29746667
Proper citation: RRID:Addgene_109428 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109423 Copy
Species: Synthetic
Genetic Insert: nCas9n and mKate2
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Zebrafish plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109547 Copy
Species: Synthetic
Genetic Insert: mCherry T2A GFP
Vector Backbone Description: Backbone Size:9268; Vector Backbone:HIV-1 NL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29746667
Proper citation: RRID:Addgene_109427 Copy
Species: Synthetic
Genetic Insert: mCarmine
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30220597
Comments: mCarmine is a far red emitting fluorescent protein (excitation: 603; emission: 675)
Proper citation: RRID:Addgene_109484 Copy
Species: Synthetic
Genetic Insert: actc1b-mKate2-GPI-pA
Vector Backbone Description: Vector Backbone:actc1b-ccdb; Vector Types:Zebrafish plasmids; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109490 Copy
Species: Synthetic
Genetic Insert: actc1b-Lifeact-mKate2
Vector Backbone Description: Vector Backbone:pDEST-actc1b-ccdb-mKate2; Vector Types:Zebrafish plasmids; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109493 Copy
Species: Synthetic
Genetic Insert: actc1b- mKate2-KDEL-pA
Vector Backbone Description: Vector Backbone:actc1b-ccdb; Vector Types:Zebrafish plasmids; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109494 Copy
Species: Synthetic
Genetic Insert: mCarmine
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30220597
Comments: mCarmine is a far red emitting fluorescent protein (excitation: 603; emission: 675)
Proper citation: RRID:Addgene_109486 Copy
Species: Synthetic
Genetic Insert: actc1b-mKate2-CaaX(tK)
Vector Backbone Description: Vector Backbone:pDEST-actc1b-mKate2-ccdb; Vector Types:Zebrafish plasmids; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109488 Copy
Species: Synthetic
Genetic Insert: Tol2 Transposase
Vector Backbone Description: Vector Backbone:pT3TS; Vector Types:Zebrafish plasmids; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109768 Copy
Species: Synthetic
Genetic Insert: L4
Vector Backbone Description: Vector Backbone:pET30; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27548700
Proper citation: RRID:Addgene_110068 Copy
Species: Synthetic
Genetic Insert: L2
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27548700
Proper citation: RRID:Addgene_110067 Copy
Species: Synthetic
Genetic Insert: ENH_BsmB1-MCS-Avi
Vector Backbone Description: Backbone Size:4263; Vector Backbone:pTK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33111104
Comments: Contains BsmBI LacZ cassete for enhancer of choice cloning by Golden gate. Protein of interest can be inserted at the NcoI and SnaBI restriction sites.
Proper citation: RRID:Addgene_110205 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29779950
Proper citation: RRID:Addgene_110135 Copy
Species: Synthetic
Genetic Insert: mito-GCaMP5
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pCFJ210; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25313960
Proper citation: RRID:Addgene_110137 Copy
Species: Synthetic
Genetic Insert: Brainbow1.0L
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pKS-ins:MCS; cryaa:RFP; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939870
Comments: Brainbow1.0L fragment obtained from Addgene Plasmid Number: 18725 (Thy1-Brainbow-1.0 L).
Proper citation: RRID:Addgene_110283 Copy
Species: Synthetic
Genetic Insert: gHH_44
Vector Backbone Description: Backbone Size:5919; Vector Backbone:pCDB364; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30152054
Proper citation: RRID:Addgene_110276 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:#26655; Backbone Size:6783; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110409 Copy
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