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  • Bacterial Resistance:chloramphenicol (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pUdO3c
 
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RRID:Addgene_210232 Chloramphenicol PMID:38235654 Backbone Marker:Host-Microbe Interactions lab; Vector Backbone:pUdO3c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:48 0
pZa pBAD csgA CBDcex pLac Ara csgGEF
 
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RRID:Addgene_226378 csgA and csgG,E,F genes of curli operon Cellulomonas fimi Chloramphenicol PMID:39137519 Backbone Size:6211; Vector Backbone:pZa; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:49 0
ICMAB - pET22b T7 EncFtn Mms6
 
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RRID:Addgene_225538 Iron binding protein Magnetospirillum magneticum AMB-1 strain Chloramphenicol PMID:35567331 Backbone Size:5289; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:49 0
pCCI-YFVΔCME-mScarlet
 
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RRID:Addgene_233589 YFV-17D lacking C, prM, E but with mScarlet Synthetic Chloramphenicol Please view the attached protocol for growing plasmid in the lab. If protocol is not followed, it is likely DNA preps will contain a significant number of DNA impurities. Backbone Size:8888; Vector Backbone:PCCI; Vector Types:Transneural tracing; Bacterial Resistance:Chloramphenicol Lacking C, prM, and E 2026-08-15 01:32:04 0
pZP06C
 
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RRID:Addgene_232322 mCherry Discosoma sp. Chloramphenicol This plasmid contains mutations in sacB, however, it has been verified to function for counterselection when sucrose is present. Vector Backbone:pZP06B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:32:07 0
pLenti-uORF1-MET
 
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1+ mentions
RRID:Addgene_214104 MET 5'UTR Homo sapiens Chloramphenicol PMID:39774381 Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol uORF1 mutation 2026-08-15 01:31:37 1
pLenti_5'UTR_MET_GFP
 
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1+ mentions
RRID:Addgene_214103 MET 5'UTR Homo sapiens Chloramphenicol PMID:39774381 Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:37 1
pM-LSSmOrange
 
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RRID:Addgene_228619 LSSmOrange Synthetic Chloramphenicol PMID:40326708 Please visit https://doi.org/10.1101/2024.10.23.619855 for bioRxiv preprint. Backbone Marker:Birtwistle lab; Vector Backbone:pMulti; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol We introduced some silent mutations to avoid essential restriction enzyme sites. 2026-08-15 01:31:38 0
pLenti-uORF2-MET
 
Resource Report
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1+ mentions
RRID:Addgene_214105 MET 5'UTR uORF2 mutated Homo sapiens Chloramphenicol PMID:39774381 Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:39 1
pLenti-uORF1,2-MET
 
Resource Report
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1+ mentions
RRID:Addgene_214106 MET 5'UTR uORF1 and 2 mutated Chloramphenicol PMID:39774381 Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:39 1
pCRISPR-PsppA-mCherry
 
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1+ mentions
RRID:Addgene_225428 mCherry, Cas9, SgRNA E. coli Chloramphenicol PMID:39817779 Backbone Size:3150; Vector Backbone:pSIP401 (spp-based expression) derivative, lacks spp-genes for induction; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:40 1
pJBL7058
 
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RRID:Addgene_167227 J23119-AKSIRV Other Chloramphenicol Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:31:42 0
pACYCDuet-1:ChrA-C63S-C66S
 
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RRID:Addgene_225082 ChrA-C63S/C66S Chryseobacterium sp. JM1 Chloramphenicol PMID:37252350 Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol C63S/C66S 2026-08-15 01:31:42 0
pEC2796 (pSpy0C4)
 
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RRID:Addgene_220280 Chloramphenicol PMID:38911550 The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white. Resource Information: The MCS featuring the Plac_mrfp cassette and the chloramphenicol resistance cassette were taken from pSpy1C. The pUC origin for replication in E. coli was taken from pUC19-mKate2∼ssrA. Up- and downstream homologous regions of sagB were amplified from the S. pyogenes SF370 genome. Linearise the plasmid with the restriction enzyme Alw44I before transformation in S. pyogenes to favour double crossover recombination of the plasmid into the sagB locus. Positive transformants will show loss of hemolysis on TSA blood agar plates. Please download the file linked below to obtain a well annotated map of the plasmid. Backbone Size:5256; Vector Backbone:pSpy0C4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:30:27 0
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
 
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RRID:Addgene_218513 ffluc Photinus pyralis Chloramphenicol PMID:38911550 This high-copy replicative shuttle plasmid features an erythromycin-inducible riboswitch driving expression of the Firefly luciferase in the presence of erythromycin. Luminescence can be detected by adding luciferin to the culture. See associated publication for more details. Resource Information: The erythromycin-inducible riboswitch was taken from pLZ12Km2-TA:PgyrA(Sag)-ermBL-ermB':A2I:ffluc-tt3, which was used in the following publication: Dynamics of diversified A-to-I editing in Streptococcus pyogenes is governed by changes in mRNA stability Thomas F. Wulff, Karin Hahnke, Anne-Laure Lécrivain, Katja Schmidt, Rina Ahmed-Begrich, Knut Finstermeier, Emmanuelle Charpentier bioRxiv 2023.09.19.555891; doi: https://doi.org/10.1101/2023.09.19.555891 The ffluc reporter gene was taken from the original pLZ12Km2-P23R:TA:ffluc plasmid (Plasmid #88900). Please download the detailed plasmid map using the file linked below Vector Backbone:pSpy1C; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol deletion of the Plac_mrfp cassette 2026-08-15 01:30:27 0
pEC2483 (pSpy1C)
 
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RRID:Addgene_220248 Chloramphenicol PMID:38911550 The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white. Resource Information: We took the rolling circle replicon pSH71 and the chloramphenicol resistance cassette from pIB166 (Plasmid #90189). The the Plac_mrfp cassette for the MCS is derived from pBS3Clux (Plasmid #55172). Please download the file linked below to obtain a well annotated map of the plasmid. Backbone Size:4917; Vector Backbone:pSpy1C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:30:27 0
LCB3_v1.2 (MTK 3a) (pZYW045)
 
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RRID:Addgene_194228 LCB3_v1.2 Synthetic Chloramphenicol PMID:33907743 Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint. Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:30:32 0
EF1a Promoter (MTK 2) (pAN2824)
 
Resource Report
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RRID:Addgene_194225 EF1a Promoter Homo sapiens Chloramphenicol PMID:33907743 Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint. Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:30:32 0
LCB1_v1.3 (MTK 3a) (pZYW044)
 
Resource Report
Resource Website
RRID:Addgene_194227 LCB1_v1.3 Synthetic Chloramphenicol PMID:33907743 Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint. Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:30:32 0
pHBJT004
 
Resource Report
Resource Website
RRID:Addgene_225155 Chloramphenicol PMID:39244484 Mid copy plasmid (p15a ori) with pUC18 MCS with FLAG epitope integrated at SmaI site, confers chloramphenicol resistance Backbone Size:2340; Vector Backbone:pSU18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:30:32 0

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