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Species: Homo sapiens
Genetic Insert: SMAD4
Vector Backbone Description: Vector Backbone:pLVX-IRES-Hyg; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26898331
Proper citation: RRID:Addgene_107128 Copy
Species: Homo sapiens
Genetic Insert: RB del22
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Comments: EcoRI is an internal site as well. See author's map. To learn more about del22 mutant, see reference: Sellers WR, Rodgers JW, and Kaelin WG, PNAS 92:11544.
Proper citation: RRID:Addgene_10721 Copy
Species: Homo sapiens
Genetic Insert: RB
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Proper citation: RRID:Addgene_10720 Copy
Species: Synthetic
Genetic Insert: ecAT3.10
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5000; Vector Backbone:pCMV(MinDis) [variant of pDisplay]; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29121644
Proper citation: RRID:Addgene_107215 Copy
Species: Other
Genetic Insert: EF2132
Vector Backbone Description: Backbone Size:5750; Vector Backbone:pCN-50; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29463653
Proper citation: RRID:Addgene_107191 Copy
Species: Synthetic
Genetic Insert: 1D3-28Z. 1-3 mut
Vector Backbone Description: Backbone Size:5586; Vector Backbone:MSGV1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20631379
Proper citation: RRID:Addgene_107227 Copy
Species: Synthetic
Genetic Insert: 1D3-28Z All ITAMs intact
Vector Backbone Description: Backbone Size:5584; Vector Backbone:MSGV1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20631379
Proper citation: RRID:Addgene_107226 Copy
Genetic Insert: KKH SaCas9
Vector Backbone Description: Backbone Marker:Richard Sherwood Lab; Backbone Size:6637; Vector Backbone:p2T-CAG-MCS-BlastR; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30405244
Proper citation: RRID:Addgene_107189 Copy
Species: Other
Genetic Insert: aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29335603
Proper citation: RRID:Addgene_107313 Copy
Species: Other
Genetic Insert: EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29335603
Proper citation: RRID:Addgene_107311 Copy
Species: Other
Genetic Insert: dSV40-dCas9-10xGCN4
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29335603
Proper citation: RRID:Addgene_107310 Copy
Species: S. aureus
Genetic Insert: SaCas9
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107317 Copy
Species: Homo sapiens
Genetic Insert: RINS1
Vector Backbone Description: Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28366620
Proper citation: RRID:Addgene_107290 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107320 Copy
Genetic Insert: RNA motif plasmid cloning backbone
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: Digest plasmid with BsmBI and ligate RNA motif of interest. The RNA motif of interest would be in the 3UTR and flanked by BoxB motifs.
Oligo cloning can be performed similarly to sgRNA cloning. Primers to order:
Primers (5'->3')
F GCTT
Proper citation: RRID:Addgene_107253 Copy
Species: Homo sapiens
Genetic Insert: 6x Halo-EGFP- 2x PACT
Vector Backbone Description: Vector Backbone:pERB219; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29097549
Proper citation: RRID:Addgene_107265 Copy
Species: Synthetic
Genetic Insert: ProA-RBS(B0032)-Gemini (E0051)
Vector Backbone Description: Backbone Marker:http://parts.igem.org/Part:pSB3C5; Backbone Size:2738; Vector Backbone:pSB3C5; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20843779
Proper citation: RRID:Addgene_107241 Copy
Genetic Insert: MARK3
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107235 Copy
Genetic Insert: toehold 7 + sfGFP
Vector Backbone Description: Backbone Marker:New England Biolabs (cloning vector); Backbone Size:2202; Vector Backbone:pNP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30083608
Proper citation: RRID:Addgene_107355 Copy
Species: Synthetic
Genetic Insert: BASU
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: The BASU protein sequence has 4 mutations relative to wild type Bacillus subtilis biotin ligase (Uniprot P0CI75): N terminal truncation of 65 aa, R124G, E323S and G325R.
Proper citation: RRID:Addgene_107250 Copy
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