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Species: Other
Genetic Insert: loxP-hyg-cat-loxP
Vector Backbone Description: Backbone Marker:Jeffery Murry - Eric Rubin Lab; Vector Backbone:pJM1; Vector Types:Bacterial Expression, Source of loxP-hyg-cat- loxP for PCR amplification.; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25779316
Comments: This vector contains a hyg-cat cassette, flanked by loxP sites, that can be used for the construction of recombineeng substrates for M. smegmatis or M. tuberculosis. It contains a 1 bp change (relative to starting plasmid pJM1) at position 2008 (G to C) that eliminate a potential stop codon that could interfere with expression of downstream functions following excision of the cassette from the chromosome by Cre recombinase.
The hygromycin resistance gene encodes M13I and I111T variants compared to the NCBI reference [ADL66925.1]. These variants should not interfere with function.
Proper citation: RRID:Addgene_71487 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Ras GTPase
Proper citation: RRID:Addgene_54243 Copy
Species: Other
Genetic Insert: Peroximal Targeting Signal 1 (PTS1)
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mEmerald-Peroxisomes; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Peroximal Targeting Signal 1 (PTS1). Excitation = 487; Emission = 509. Localization data: Peroxisomes
Proper citation: RRID:Addgene_54228 Copy
Species: Other
Genetic Insert: mCherry and EGFP
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBS SK(+); Vector Types:Mammalian Expression, Mouse Targeting, CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28421278
Proper citation: RRID:Addgene_54322 Copy
Species: Other
Genetic Insert: cauliflower mosaic virus 35S promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54409 Copy
Species: Other
Genetic Insert: L. japonicus polyubiquitin promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54408 Copy
Species: Other
Genetic Insert: cauliflower mosaic virus 35S promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54406 Copy
Species: Other
Genetic Insert: Terminator of Cauliflower mosaic virus CaMV
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54442 Copy
Species: Other
Genetic Insert: ccdB A-B
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54459 Copy
Species: Other
Genetic Insert: lacZ C-D
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54458 Copy
Species: Other
Genetic Insert: FTR
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54902 Copy
Species: Other
Genetic Insert: CytERM
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cytochrome p450 contains V8G and H24Y mutations. These mutations should not affect function.
Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55024 Copy
Species: Other
Genetic Insert: SV40 T-Antigen Nuclear Localization Signal (NLS)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55110 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55126 Copy
Species: Other
Genetic Insert: CytERM
Vector Backbone Description: Backbone Size:4750; Vector Backbone:EBFP2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cytochrome p450 . Excitation = 383; Emission = 448
Proper citation: RRID:Addgene_55238 Copy
Species: Other
Genetic Insert: SV40 T-Antigen Nuclear Localization Signal (NLS)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:EBFP2-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Nucleus = NLS. Excitation = 383; Emission = 448
Proper citation: RRID:Addgene_55249 Copy
Species: Other
Genetic Insert: CytERM
Vector Backbone Description: Backbone Size:4750; Vector Backbone:MiCy-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cytochrome p450 . Excitation = 472; Emission = 495
Proper citation: RRID:Addgene_55338 Copy
Species: Other
Genetic Insert: SV40 T-Antigen Nuclear Localization Signal (NLS)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:ECFP-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Nucleus = Nuc. Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55346 Copy
Species: Other
Genetic Insert: CytERM
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean3-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21479270
Comments: Cytochrome p450 . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55412 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55388 Copy
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