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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
CIT1-2EGFP-ter-URA3
 
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RRID:Addgene_184767 CIT1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:08 0
AFG3-2EGFP-ter-URA3
 
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RRID:Addgene_184770 AFG3 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:08 0
OM14-2EGFP-UG74-ter
 
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RRID:Addgene_184758 OM14 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:05 0
kanMX6-ins2
 
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1+ mentions
RRID:Addgene_195039 KanR Saccharomyces cerevisiae Ampicillin PMID:36503721 This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference. For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation" The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA. Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:25:07 1
TRP1MX6-ins4
 
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RRID:Addgene_195041 TRP1 Saccharomyces cerevisiae Ampicillin PMID:36503721 This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference. For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation" The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA. Backbone Size:2417; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:25:07 0
kanMX6-ins6
 
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RRID:Addgene_195043 KanR Saccharomyces cerevisiae Ampicillin PMID:36503721 This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference. For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation" The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 sequence was amplified from S. cerevisiae gDNA. Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:25:10 0
14x PP7 with loxP-kanMX-loxP
 
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1+ mentions
RRID:Addgene_189939 14x PP7 with loxP-kanMX-loxP Saccharomyces cerevisiae Ampicillin PMID:31101674 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:08 1
SIVURA MCP-GFPenvy
 
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RRID:Addgene_189940 MCP-GFPEnvy Saccharomyces cerevisiae Ampicillin PMID:37824008 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:11 0
pCAS-mak10 gRNA-MAK10Δ
 
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RRID:Addgene_160384 MAK10 Saccharomyces cerevisiae Kanamycin MAK10 was deleted in an S288C background with Cre-loxP. Lox-ed mak10 "deletion " fragment" consisting of sequences upstream and downstream of deleted ORF (~800bp) was amplified, adding XhoI restriction sites, and cloned into the vector. This insert serves as a donor template for making a MAK10 deletion. The vector is an all-in-one gRNA/Cas9/donor template vector for making a marker-free MAK10 deletion in yeast strains Backbone Size:8743; Vector Backbone:pCAS-mak10 gRNA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:25:11 0
SIV URA PCP-NLS-GFPEnvy
 
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1+ mentions
RRID:Addgene_189941 PCP-NLS-GFPEnvy Saccharomyces cerevisiae Ampicillin PMID:37127821 Please visit https://www.biorxiv.org/content/10.1101/2021.12.15.472793v1 for bioRxiv preprint. Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:08 1
pET PI-SceI C-His K403A
 
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RRID:Addgene_195612 PI-SceI Saccharomyces cerevisiae Ampicillin PMID:9804821 Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag and a K403A mutation in the PI-SceI ORF. Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin A1463G, A1464C 2026-08-15 01:25:08 0
pSPIH6
 
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RRID:Addgene_190676 Small ubiquitin-like modifier Saccharomyces cerevisiae Ampicillin PMID:37020212 Please hold for publication. Backbone Marker:NEB; Backbone Size:6706; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin None 2026-08-15 01:25:09 0
UG75-CDS1-3HA
 
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RRID:Addgene_184760 CDS1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:12 0
UG75-PAH1-3HA
 
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RRID:Addgene_184761 PAH1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:15 0
UG75-DGK1-3HA
 
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RRID:Addgene_184759 DGK1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:12 0
pOBD2 Pab1 WT (123-204)
 
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RRID:Addgene_196834 Pab1 Saccharomyces cerevisiae Ampicillin PMID:25671604 pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment. Expresses Pab1 fragment fused to GAL4BD. Backbone Size:7167; Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 123-204 amino acids 2026-08-15 01:25:59 0
pROS_phleo-Tps1/Gsy2
 
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RRID:Addgene_196612 TPS1, GSY2 Saccharomyces cerevisiae Ampicillin PMID:36849832 Cloning details are in the Methods section and Supplementary Table 2 of the paper. Vector Backbone:pROS13; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:26:02 0
UG75-ADH1-DGK1-3HA
 
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RRID:Addgene_198595 DGK1 Saccharomyces cerevisiae Ampicillin PMID:32859196 Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:26:04 0
p415 Ret2
 
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RRID:Addgene_73872 Ret2 Saccharomyces cerevisiae Ampicillin PMID:27298352 Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:19:32 0
P415 Ret2LD
 
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RRID:Addgene_73875 Ret2LD Saccharomyces cerevisiae Ampicillin PMID:27298352 Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:19:32 0

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