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Species: Saccharomyces cerevisiae
Genetic Insert: CIT1
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184767 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AFG3
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OM14
Vector Backbone Description: Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184758 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195039 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2417; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 sequence was amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195043 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 14x PP7 with loxP-kanMX-loxP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31101674
Proper citation: RRID:Addgene_189939 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-GFPEnvy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37824008
Proper citation: RRID:Addgene_189940 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MAK10
Vector Backbone Description: Backbone Size:8743; Vector Backbone:pCAS-mak10 gRNA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: MAK10 was deleted in an S288C background with Cre-loxP. Lox-ed mak10 "deletion " fragment" consisting of sequences upstream and downstream of deleted ORF (~800bp) was amplified, adding XhoI restriction sites, and cloned into the vector. This insert serves as a donor template for making a MAK10 deletion. The vector is an all-in-one gRNA/Cas9/donor template vector for making a marker-free MAK10 deletion in yeast strains
Proper citation: RRID:Addgene_160384 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PCP-NLS-GFPEnvy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37127821
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.12.15.472793v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_189941 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9804821
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag and a K403A mutation in the PI-SceI ORF.
Proper citation: RRID:Addgene_195612 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Small ubiquitin-like modifier
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6706; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37020212
Comments: Please hold for publication.
Proper citation: RRID:Addgene_190676 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDS1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184760 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PAH1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184761 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DGK1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184759 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pab1
Vector Backbone Description: Backbone Size:7167; Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25671604
Comments: pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment. Expresses Pab1 fragment fused to GAL4BD.
Proper citation: RRID:Addgene_196834 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TPS1, GSY2
Vector Backbone Description: Vector Backbone:pROS13; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Cloning details are in the Methods section and Supplementary Table 2 of the paper.
Proper citation: RRID:Addgene_196612 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DGK1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_198595 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73872 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73875 Copy
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