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Genetic Insert: SO_2192
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function
Proper citation: RRID:Addgene_125070 Copy
Genetic Insert: SO_0621
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function
Proper citation: RRID:Addgene_125069 Copy
Species: Other
Genetic Insert: optimized RBS
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pFD116 with an optimized RBS to use in E. coli; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_125546 Copy
Species: Synthetic
Genetic Insert: nScCas9-ABE7.10, wheat/human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125820 Copy
Species: Synthetic
Genetic Insert: A3A-PBE, wheat codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125818 Copy
Species: Synthetic
Genetic Insert: nSpCas9-ABE7.10 base changer, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125810 Copy
Species: Synthetic
Genetic Insert: FnCas12a, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125811 Copy
Species: Synthetic
Genetic Insert: LbCas12a, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125812 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125809 Copy
Species: Synthetic
Genetic Insert: LbCas12a, rice codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125803 Copy
Species: Synthetic
Genetic Insert: FnCas12a, rice codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125801 Copy
Species: Synthetic
Genetic Insert: SaCas9, Arabidopsis codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125799 Copy
Species: Synthetic
Genetic Insert: Level0 Rice OsAct1 Pol II promoter
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125882 Copy
Species: Synthetic
Genetic Insert: Level0 Parsley PcUbi4-2 Pol II promoter
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125880 Copy
Species: Synthetic
Genetic Insert: Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125881 Copy
Species: Synthetic
Genetic Insert: Taz
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29650958
Proper citation: RRID:Addgene_104333 Copy
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30083222
Comments: There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.
Proper citation: RRID:Addgene_104438 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC19
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104684 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC15
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104680 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC16
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104681 Copy
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