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Species: Other
Genetic Insert: CRE
Vector Backbone Description: Backbone Size:10785; Vector Backbone:AAVS1-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33765444
Proper citation: RRID:Addgene_165457 Copy
Species: Homo sapiens
Genetic Insert: PPAR gamma
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10555149
Comments: Note that there are some discrepancies between the insert in this plasmid and the canonical GenBank PPARgamma. The Vogelstein lab maintains that this plasmid worked as specified when they made and used it.
Proper citation: RRID:Addgene_16549 Copy
Species: Homo sapiens
Genetic Insert: p73
Vector Backbone Description: Backbone Size:5210; Vector Backbone:pBI-MCS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588737
Comments: Tet-responsive p73 expression construct.
Proper citation: RRID:Addgene_16545 Copy
Vector Backbone Description: Backbone Size:5210; Vector Backbone:pBI-MCS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588737
Comments: The tet-responsive reporter plasmid pBI-EGFP was purchased from Clontech and modified by the inclusion of a polylinker to create pBI-MCS-EGFP.
Proper citation: RRID:Addgene_16542 Copy
Genetic Insert: tTA
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pMk10-59; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588737
Comments: tTA is a a tetracycline-controlled transactivator generated by fusing the tet repressor with the activating domain of virion protein 16 of herpes simplex virus. tTA-IRES-Neo was generated by cloning PCR-amplified tTA cDNA from pUHD15-1 (Gossen, M. & Bujard, H., 1992, PNAS 89, 5547-5551) into the XbaI site of plasmid pMk10-59 (Kobayashi, M et al., 1996, Biotechniques 21, 398-402).
Proper citation: RRID:Addgene_16541 Copy
Species: Homo sapiens
Genetic Insert: Human histone H2B
Vector Backbone Description: Backbone Marker:-; Backbone Size:8023; Vector Backbone:pRRL-cPPT-hPGKTMPrtTA-WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29944140
Comments: ABOUT BACKBONE:
The lentiviral backbone was originally designed and constructed by Barde I et al. (Barde I et al. Mol Ther. 2006. 13(2): 382-90. PMID: 16275162).
ABOUT TEST DIGESTION:
Since Xba I restriction site is lost during cloning, we recommend checking the plasmid using the enzymes: Xho I and Nhe I. You should get two fragments around 1600 bp (insert+vector) and 7500 bp (vector).
Proper citation: RRID:Addgene_165494 Copy
Genetic Insert: ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pB1H2_UV2-ω-dCas9::K2_UV5-sgRNA; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33441553
Proper citation: RRID:Addgene_165606 Copy
Species: Homo sapiens
Genetic Insert: TCF-4 binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10749138
Comments: TCF-4 mutant binding sequence - CCTTTGGC
Proper citation: RRID:Addgene_16559 Copy
Species: Homo sapiens
Genetic Insert: MADMYC
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: MADMYC: the amino
terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional
repression domain (aa 1-38, the Sin3-interaction region) of Mad.
Proper citation: RRID:Addgene_16557 Copy
Species: Viral
Genetic Insert: SR39TK
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165841 Copy
Species: P1 bacteriophage
Genetic Insert: cre recombinase
Vector Backbone Description: Backbone Size:7700; Vector Backbone:na; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11371342
Comments: Cre expression vector.
Proper citation: RRID:Addgene_16583 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACCT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CCTGTGATAG CAACAGATCA CCTGGCTGCC ATCGCCCCTC-3' [Oligo C , for mutant MBS1-3];
5'-ATGAATTCCG GACGTTCTGG GCAGGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCCAGG-3' [Oligo D, for mutant MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16565 Copy
Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD130.04, Ligation number L4506.
Proper citation: RRID:Addgene_1658 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACGT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CGTGTGATAG CAACAGATCA CGTGGCTGCC ATCGCCCCTC-3' [Oligo A, for wild-type c-MYC binding sites (MBS)1-3]; 5'-ATGAATTCCG GACGTTCTGG GCACGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCC-3' [Oligo B, for wild-type MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16564 Copy
Species: Homo sapiens
Genetic Insert: BAX knockout
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11062132
Comments: This targeting construct consists of two homologous arms and a hygromycin-resistant gene.
Proper citation: RRID:Addgene_16575 Copy
Species: Other
Genetic Insert: HCoV-OC43 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166015 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166019 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166018 Copy
Species: Other
Genetic Insert: HCoV-229E S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166016 Copy
Species: Other
Genetic Insert: SARS-CoV-2 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166010 Copy
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