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Species: Synthetic
Genetic Insert: Rncp-iGluSnFR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5268; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29308878
Comments: Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.
Proper citation: RRID:Addgene_107336 Copy
Species: Homo sapiens
Genetic Insert: RB del663
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Comments: del663 created by site-directed mutagenesis using this primer: GCCTATCTCCGGCTAaatgctgctatacgatcgCTTCTGTCTGAGCAC
Proper citation: RRID:Addgene_10728 Copy
Species: Homo sapiens
Genetic Insert: RB 567L
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Comments: 567L created by site-directed mutagenesis using this primer: GCATGGCTCTCAGATcttCCTTTATTTGATCTT
Proper citation: RRID:Addgene_10725 Copy
Species: Homo sapiens
Genetic Insert: UBE3A
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWB-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28003368
Proper citation: RRID:Addgene_107349 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting PBRM1
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain (Addgene plasmid # 21915); Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082722
Proper citation: RRID:Addgene_107399 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting CRBN
Vector Backbone Description: Vector Backbone:plenti-px330-pGK-Pur; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24292623
Proper citation: RRID:Addgene_107383 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting CRBN
Vector Backbone Description: Vector Backbone:plenti-px330-pGK-Pur; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24292623
Proper citation: RRID:Addgene_107382 Copy
Species: Homo sapiens
Genetic Insert: cereblon
Vector Backbone Description: Vector Backbone:plenti-UBC-HA-gate-pGK-HYG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24292623
Proper citation: RRID:Addgene_107378 Copy
Species: Homo sapiens
Genetic Insert: alpha-synuclein
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25657004
Proper citation: RRID:Addgene_107425 Copy
Species: Rattus norvegicus
Genetic Insert: calcium channel beta2a auxillary subunit
Vector Backbone Description: Backbone Size:5163; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9705988
Comments: We received it in pBluescript and subcloned it into pMT2 plasmid.
Proper citation: RRID:Addgene_107424 Copy
Species: Homo sapiens
Genetic Insert: IKZF1-V1
Vector Backbone Description: Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24292623
Proper citation: RRID:Addgene_107387 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Proper citation: RRID:Addgene_10745 Copy
Species: Homo sapiens
Genetic Insert: control gRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082722
Proper citation: RRID:Addgene_107402 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting PBRM1
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain (Addgene plasmid # 21915); Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082722
Proper citation: RRID:Addgene_107400 Copy
Species: Homo sapiens
Genetic Insert: PBRM1
Vector Backbone Description: Vector Backbone:pTRIPZ-neo; Vector Types:Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28082722
Comments: This plasmid is unstable and likely to recombine when grown. Upon receipt it is essential to screen multiple colonies to confirm the intact plasmid is present. Recombined products are not expected to impact viral packaging if the full length plasmid is present.
Proper citation: RRID:Addgene_107406 Copy
Species: Escherichia coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29361543
Proper citation: RRID:Addgene_107455 Copy
Species: Escherichia Coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29361543
Proper citation: RRID:Addgene_107454 Copy
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A modified version of pSG5 (from Stratagene) in which a new polylinker has been cloned in.
Proper citation: RRID:Addgene_10737 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Proper citation: RRID:Addgene_10739 Copy
Vector Backbone Description: Backbone Marker:David Root; Backbone Size:8234; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: GFP can be used as an N-terminal tag, but the GFP can also be eliminated and used as a 'dropout' for cloning into the vector. NheI can be used to drop the GFP, and SalI can be used to keep GFP as an N-terminal tag. Its quite useful to have GFP as a tag as it can be used for localization, immunoprecipitation, and for Western Blotting.
Proper citation: RRID:Addgene_107551 Copy
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