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Species: Synthetic
Genetic Insert: EGFP-2A-Tetanus Toxin
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166611 Copy
Species: Synthetic
Genetic Insert: ChR2-TdTomato
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166610 Copy
Species: Synthetic
Genetic Insert: TurboID
Vector Backbone Description: Vector Backbone:pDONR p2r-p3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_166570 Copy
Species: Synthetic
Genetic Insert: BASU
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_166565 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166601 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166606 Copy
Species: Synthetic
Genetic Insert: EGFP-2A-Tetanus Toxin
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166603 Copy
Species: Synthetic
Genetic Insert: rtTA
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166598 Copy
Species: Synthetic
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166597 Copy
Species: Synthetic
Genetic Insert: hM3Dq-mCherry
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166599 Copy
Species: Synthetic
Genetic Insert: ActA tail anchor (Listeria monocytogenes)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: "SGLRSRGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDAR
GSGLRSRAQASNSAVDARV" amino acid targeting sequence, which we refer to as "ActA", for the mitochondrial outer membrane. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. "79aa" indicates the 79 amino acid flexible linker region between Venus and the ActA targeting signal.
Proper citation: RRID:Addgene_166763 Copy
Species: Synthetic
Genetic Insert: dCas12a-NLS
Vector Backbone Description: Backbone Size:7206; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34197613
Proper citation: RRID:Addgene_166731 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: Plasmid can be used to express monomeric Venus fluorophore in bacteria. We used E.coli BL21-AI (Arabinose induction) for protein production, induced 5h at 30oC. Purify protein via Nickel column chromatography. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166756 Copy
Species: Synthetic
Genetic Insert: PE2-NG variant
Vector Backbone Description: Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446856
Proper citation: RRID:Addgene_176933 Copy
Species: Synthetic
Genetic Insert: moxGFP-P2A-T2A
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5857; Vector Backbone:pSBbi-Pur (Addgene Plasmid #60523); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34877484
Comments: The moxGFP-P2A-T2A construct was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]). It was codon optimized for human and mouse expression and includes two additional cloning sites (5' EcoRV - 3' SalI and 5' SmaI - 3' XbaI, see the map uploaded by the depositor) for the polycistronic expression of up to three genes: moxGFP and two more. This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate mammalian cells with stable expression of the genes of interest. Enjoy!
Proper citation: RRID:Addgene_176893 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6091; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177163 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6091; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177162 Copy
Species: Synthetic
Genetic Insert: yeCherry
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6088; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177165 Copy
Species: Synthetic
Genetic Insert: yeCherry
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6088; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177164 Copy
Species: Synthetic
Genetic Insert: PCP-VP64
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:7364; Vector Backbone:pRS42K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Proper citation: RRID:Addgene_177156 Copy
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