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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Bcr/Abl P190-pLEF Resource Report Resource Website |
RRID:Addgene_38159 | BCR/ABL P190 | Homo sapiens | Ampicillin | PMID:18070886 | Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.75 kb BamHI/Sal I + 3' 6.3 kb Sal I/EcoRI BCR/ABL fragments into pLEF digested with BamHIxEcoRI. The ABL part of the insert contains the natural TAG and 3' UT sequences. Addgene NGS identified a sequence discrepancy at nucleotide 3168 resulting in a T117M mutation in the ABL-1 sequence, relative to Genbank ID NP_005148.2 and Addgene Plasmid #38158. This is not a known polymorphism and the effect of this, if any, on the protein is not known. | Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | T117M in c-ABL1 | 2026-08-15 01:14:19 | 0 |
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EYFP-Abr in pCCL-cppt178-MNDU3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38155 | Abr | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Jess Cunnick. Abr was first subcloned into pEYFP-C1 Bgl II x KpnI as a 0.4 kb 5' BamHI/BstEII + 2.2 kb BstEII- KpnI fragment. The EYFP-Abr fusion was isolated as a 5' AgeI- 3' KpnI fragment and inserted into pCCL-cppt178-MNDU3-X3 digested with Xma I x Kpn I. | Backbone Marker:Don Kohn [email protected]; Backbone Size:6600; Vector Backbone:pCCL-cppt178-MNDU3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 1 | |
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Abr-pLEF Resource Report Resource Website |
RRID:Addgene_38157 | ABR | Homo sapiens | Ampicillin | PMID:18070886 | Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.4 kb BamHI/BstEII + 3' 2.2 kb BstEII/EcoRI ABR fragments into pLEF digested with BamHIxEcoRI. The ABR insert contains the natural TAG and 3' UT sequences. Addgene sequencing found an E24G point mutation--this does not alter plasmid function. | Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | |
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GST-Bcr pLEF Resource Report Resource Website |
RRID:Addgene_38160 | Bcr | Homo sapiens | Ampicillin | PMID:14614449 | Constructed by Anja Reichert. Generated in a 3-way ligation consisting of 5' 0.6 kb BamHI/SalI + 3' 3.5 kb SalI/XbaI BCR fragments into pLEF digested with BamHIxXbaI. The BCR insert contains the natural TAG and 3' UT sequences. At the 3' end there are polylinker sequences from pSK. | Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | |
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Bcr/Abl P210 in pAcG2T Resource Report Resource Website |
RRID:Addgene_38161 | Bcr/Abl P210 | Homo sapiens | Ampicillin | Constructed by Nora Heisterkamp. Made using a 3-way ligation with 0.6 kb 5' BamHI-SalI from BCR + 3' 7.5 kb Sal I-EcoRI into pAcG2T. Not evaluated for protein production. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
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pEGFP-N1-MITF-A Resource Report Resource Website 1+ mentions |
RRID:Addgene_38132 | MITF-A | Homo sapiens | Kanamycin | PMID:22692423 | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | deleted stop codon | 2026-08-15 01:14:19 | 9 | |
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pEBB HA cIAP1 H588A Resource Report Resource Website 1+ mentions |
RRID:Addgene_38233 | cIAP1 H588A | Homo sapiens | Ampicillin | PMID:19243308 | Backbone Marker:Baltimore Lab; Backbone Size:5350; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | H588A | 2026-08-15 01:14:20 | 1 | |
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HA-tagged Bcr Resource Report Resource Website 1+ mentions |
RRID:Addgene_38189 | BCR | Homo sapiens | Ampicillin | Constructed by Young Jin Cho. A human BCR cDNA was inserted into pKH3 (Addgene plasmid #12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (1.7 kb BamHI-SstI + 2.6 kb SstI-EcoRI). The 1.7 kb BamHI-SstI fragment was isolated from Bcr in pAcG2T (AddGene plasmid #38163). The 5’ sequence is GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG-start BCR. | Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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BCR/ABL P190 transgenic construct Resource Report Resource Website 1+ mentions |
RRID:Addgene_38185 | BCR/ABL P190 | Homo sapiens | Ampicillin | PMID:2179728 | The DNA construct was made by Guido Jenster and Nora Heisterkamp in several cloning and subcloning steps. The transgenic construct contains human BCR exon 1, intron sequences of human BCR and ABL genes, human ABL exon 2 and ABL cDNA sequences. The transgene expression is controlled by a 200-bp Sst I- Bgl II fragment of the murine metallothionein-1 (Mt1) promoter lacking glucocorticoid-responsive elements (PMID 8541551). This BCR/ABL fusion gene produces a P190 protein and is a model for Philadelphia chromosome-positive acute lymphoblastic leukemia (ALL) in humans, which is caused by a chromosomal translocation that fuses the ABL proto-oncogene with breakpoint cluster region (BCR) sequences. Leukemia development is relatively rapid on inbred C57Bl/6J and CBA backgrounds and is slower on mixed genetic backgrounds. Mice transgenic for this construct on an inbred C57Bl/6J background are also available from Jackson labs stock 017833. The insert can be separated from the vector by digestion with Sst II. | Backbone Size:3000; Vector Backbone:pSK; Vector Types:construct for making transgenic mice; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 3 | |
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human CRKL transgenic construct Resource Report Resource Website |
RRID:Addgene_38186 | CRKL | Homo sapiens | Ampicillin | PMID:11245441 | This construct was made by Arnoud van Wijk. It can be used to make transgenic mice. The DNA construct contains 7.5 kb of human CRKL locus 5’ sequences including the promoter of the human v-crk sarcoma virus CT10 oncogene homolog (CRKL) gene, joined to CRKL exon 1. Part of the intron between exons 1 and 2 encompassing around 8-kb is included and exons 2-3 as cDNA. The entire insert is around 17 kb and can be isolated free from plasmid sequences in a Kpn I x Mlu I digestion. Jackson labs stock no. 017834 is one of our CRKL transgenics containing approximately 5 copies of this transgene. CRKL overexpressing transgenics are viable but mice with high copy numbers have reduced fertility. How the CRKL transgenic construct was made: (verbatim from PMID 11245441): Human genomic clone 70 (PMID 7905853) contains 12 kb of 5’ sequences, exon 1, and 6.6 kb of intron 1 of the CRKL gene. A 350-bp RsaI fragment isolated from a CRKL cDNA (GenBank accession no. X59656) was used as a probe to specifically isolate a phage clone, CR-4, containing CRKL exon 2 flanked by 6.5 kb of intron 1 and 9.5 kb of intron 2. CRKL is located on human chromosome 22 and has been entirely sequenced; introns 1 and 2 are both around 15.5 kb. To generate a transgenic DNA construct, a 7-kb SalI-BamHI fragment from CR-4, which included a SalI site at the 5’ end from the phage polylinker and the 3’ BamHI site located in exon 2, was ligated with a 0.78-kb BamHI-EcoRI cDNA fragment, including exons 2, 3, and the 3’ untranslated region, into pSK digested with SalIxEcoRI. The insert was removed as a 7.7-kb SalI-NotI fragment and ligated with a 1.2-kb SstII-SalI fragment from intron 1 in clone 70 into pSK digested with SstII x NotI. The resulting insert was removed by digestion with SstII x NotI. The 5’ promoter and exon 1 sequences were isolated on an 8-kb EcoRI-SstII fragment that was subcloned into pSK digested with EcoRI x SstII; the insert was removed as an 8-kb KpnI-SstII fragment. The 8-kb KpnI-SstII fragment plus the 7.7-kb SstII-NotI fragment were ligated into pSL1180 digested with KpnI x NotI. | Backbone Size:3500; Vector Backbone:pSL1180; Vector Types:mouse transgenic; Bacterial Resistance:Ampicillin | Contains human CRKL introns | 2026-08-15 01:14:20 | 0 |
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pMXs-IP-EGFP-hAtg13 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38191 | autophagy-related genes 13 | Homo sapiens | Ampicillin | PMID:19211835 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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HA-tagged Abr Resource Report Resource Website |
RRID:Addgene_38190 | ABR | Homo sapiens | Ampicillin | Constructed by Young Jin Cho. A human ABR cDNA was inserted into pKH3 (Addgene plasmid 12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (0.4 kb BamHI-BstEII + 2.2 kb BstEII-EcoRI). The 5’ sequence is GGA TCC ATG-start ABR. Note: This plasmid may contain an E24G mutation -gag to ggg- introduced by Taq polymerase at an earlier cloning step. | Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 0 | ||
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pCDNA3.1Xpress-AbrGAP Resource Report Resource Website |
RRID:Addgene_38177 | Abr GAP domain | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. An 0.8 kb BamHI xEcoRI fragment from AbrGAP in pGEX3x (AddGene #36407) was inserted into pcDNA BamHI xEcoRI. The insert starts at a natural PvuII site in the ABR sequence. The fragment contains the natural TAG and 3’ UT sequences and encodes amino acid residues 597-859 (Abr isoform B). | Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | ABR GAP domain including amino acids 597-859 | 2026-08-15 01:14:19 | 0 |
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pCDNA3.1Xpress-BcrGAP Resource Report Resource Website |
RRID:Addgene_38175 | BCR | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. A 1.2 kb BamHI xEcoRI fragment from BcrGAP in pGEX3x (AddGene #36405) was inserted into pcDNA BamHI xEcoRI. Contains natural TAG and 3’ UT sequences. The construct includes amino acid residues 1004-1271. | Backbone Size:6700; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains GAP domain amino acids 1004-1271 | 2026-08-15 01:14:19 | 0 |
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pCDNA3.1Xpress-BcrGAP R1090A Resource Report Resource Website |
RRID:Addgene_38176 | BCR GAP domain encoding residues 1004-1271 with R1090A point mutation | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. pcDNA3.1Xpress-BcrGAP plasmid(AddGene #38175) was digested by BstEII x EcoRI and a 1.2 kb fragment containing the R1090 wt site, TAG and 3’ UT sequences removed. The wt fragment was replaced by a 0.6 kb BstEII x EcoRI fragment containing the R1090A mutation isolated from EGFP-BCR R1090A pEGFP (AddGene #38173). The construct contains the natural TAG and 3’ UT sequences. The difference between wt and mutant plasmids is in the 3’ UT region. The construct includes amino acid residues 1004-1271. | Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa 1004-1271 with R1090A point mutation | 2026-08-15 01:14:19 | 0 |
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Abr DH domain NE/AA pGEX Resource Report Resource Website |
RRID:Addgene_38182 | ABR DH domain N282AE283A | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. Mutagenesis done using Stratagene Quick Change kit. 0.35 kb Bgl IIxSstI fragment containing the N282A/E283A double mutation from Abr EYFP (AddGene #36419) was ligated into the "ABR DH domain pGEX" plasmid (AddGene #38181) digested with Bgl II x SstI and removal of the 0.35 kb fragment containing the wt sequence N282E283. The amino acid numbering is as in NM_021962 (isoform A). | Backbone Size:5000; Vector Backbone:pGEX 3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | ABR DH domain with N282A and E283A mutations | 2026-08-15 01:14:20 | 0 |
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Fer in pAcG2T Resource Report Resource Website 1+ mentions |
RRID:Addgene_38167 | FER | Homo sapiens | Ampicillin | Constructed by Leena Haataja. 4-way ligation of BamHI/NsiI + NsiI/KpnI + KpnI/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Fer protein but not from amplified virus stocks. Large-scale yield of protein is relatively low. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 1 | ||
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Abr in pAcG2T Resource Report Resource Website |
RRID:Addgene_38162 | ABR | Homo sapiens | Ampicillin | Constructed by Leena Haataja. 4-way ligation of BamHI/StuI + StuI/BstEII + BstEII/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Abr protein. Addgene sequencing found an E24G point mutation--this does not alter plasmid function. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
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Bcr in pAcG2T Resource Report Resource Website |
RRID:Addgene_38163 | BCR | Homo sapiens | Ampicillin | Constructed by Vesa Kaartinen. Makes Bcr protein. Nucleotide sequence from BamHI cloning site to BCR ATG is: 5' GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
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Rac1 in pAcG2T Resource Report Resource Website |
RRID:Addgene_38164 | Rac1 | Homo sapiens | Ampicillin | Constructed by Nora Heisterkamp. Rac1 cDNA isolated from K562 cDNA library. 5' BamHI site introduced by PCR. Constructed in 3-way ligation: 0.18 kb 5' BamHI-PvuII + 3' 0.4 kb PvuII-EcoRI into pAcG2T. Makes Rac1 in insect cells. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 |
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