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Species: Synthetic
Genetic Insert: PCP-VP64
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:7364; Vector Backbone:pRS42K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Proper citation: RRID:Addgene_177157 Copy
Species: Synthetic
Genetic Insert: Tandem dimer superfolder GFP
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, dre / rox ; piggyBac; Bacterial Resistance:Ampicillin
Comments: When utilized in a reporter knocked in into a genomic locus, the stop cassette in this expression vector leaked in vivo (https://doi.org/10.6084/m9.figshare.16570695.v5).
Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.
Proper citation: RRID:Addgene_177150 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting the PEAR-GFP plasmid
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35196219
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.26.441486v1.
Proper citation: RRID:Addgene_177183 Copy
Species: Synthetic
Genetic Insert: pegRNA targeting the PEAR-GFP plasmid
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35196219
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.26.441486v1.
Proper citation: RRID:Addgene_177182 Copy
Species: Synthetic
Genetic Insert: EGFP split with an intron between amino acids 95-96
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35196219
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.26.441486v1.
Proper citation: RRID:Addgene_177186 Copy
Species: Synthetic
Genetic Insert: hyperPE2
Vector Backbone Description: Backbone Size:3331; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34556671
Proper citation: RRID:Addgene_177173 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: Optimized T2A sequence: https://doi.org/10.6084/m9.figshare.13636142.v79.
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) (#137864, https://www.addgene.org/137864/) was updated with (1) improved Kozak consensus sequence, (2) a slightly different codon usage in the 5' linker of the T2A, (3) a silently mutated XhoI site in iCre was reverted, and (4) four nucleotides were restored to the 5' of the WPRE.
Proper citation: RRID:Addgene_177308 Copy
Species: Synthetic
Genetic Insert: RFP, CauNEO
Vector Backbone Description: Backbone Size:2683; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34893621
Proper citation: RRID:Addgene_177277 Copy
Species: Synthetic
Genetic Insert: DREADD Gi-mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4591; Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, INTRSECT; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177673 Copy
Species: Synthetic
Genetic Insert: DREADD Gi-mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4591; Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, INTRSECT; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177672 Copy
Species: Synthetic
Genetic Insert: KRaION1
Vector Backbone Description: Backbone Size:4002; Vector Backbone:pBAD-HisD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35427452
Comments: Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
Proper citation: RRID:Addgene_177799 Copy
Species: Synthetic
Genetic Insert: pCMV(MinDis)-iCyt_BrEt_SnFR_PM
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177747 Copy
Species: Synthetic
Genetic Insert: pCMV(MinDis)-iCytSnFR_ER
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177742 Copy
Species: Synthetic
Genetic Insert: iCytSnFR
Vector Backbone Description: Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177738 Copy
Species: Synthetic
Genetic Insert: iCyt_F_SnFR
Vector Backbone Description: Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177739 Copy
Species: Synthetic
Genetic Insert: KRaION2
Vector Backbone Description: Backbone Size:4005; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35427452
Comments: Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
Proper citation: RRID:Addgene_177802 Copy
Species: Synthetic
Genetic Insert: mSandy2
Vector Backbone Description: Backbone Marker:Twist Bioscience; Backbone Size:5234; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35222925
Proper citation: RRID:Addgene_177760 Copy
Species: Synthetic
Genetic Insert: KRaION2
Vector Backbone Description: Backbone Size:4002; Vector Backbone:pBAD-HisD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35427452
Comments: Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
Proper citation: RRID:Addgene_177800 Copy
Species: Synthetic
Genetic Insert: pAAV9-hSyn-iDianiSnFR_ER
Vector Backbone Description: Backbone Marker:Penn Vector Core; Backbone Size:5005; Vector Backbone:pAAV9-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177758 Copy
Species: Synthetic
Genetic Insert: pCMV(MinDis)-iDianiSnFR_ER
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177750 Copy
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