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Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725303.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149191 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149194 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725303.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149188 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724391.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149221 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp2
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725298.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149222 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149224 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF8 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724396.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149225 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149226 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Size:5383; Vector Backbone:pAAV-nef-CIAO; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31843925
Comments: Full name: pAAV-nef-AO-lox66-STOP-lox71-Flp-hGH-pA
Proper citation: RRID:Addgene_149294 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Size:5383; Vector Backbone:pAAV-nef-CIAO2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31843925
Comments: The CIAO2 construct replaces the lox66/71 pair used in CIAO with the lox43/44 pair to further decrease leak expression - not used in the original publication. Full name: pAAV-nef-AO-lox43-STOP-lox44-Flp-hGH-pA
Proper citation: RRID:Addgene_149296 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP-gRNA NatMX Z3EV
Vector Backbone Description: Backbone Size:1861; Vector Backbone:pTPGI; Vector Types:Insert storage (replicative in E. coli); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33191917
Proper citation: RRID:Addgene_157659 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCAS9-VP64, mCherry, KanMX
Vector Backbone Description: Backbone Size:2323; Vector Backbone:pTPGI; Vector Types:Insert storage (replicative in E. coli); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33191917
Comments: Depositor confirms mutation in mCherry and single base pair deletion in Npr2p is not a concern for plasmid function.
Proper citation: RRID:Addgene_157658 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tPcyA
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology, Optogenetics; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33522514
Proper citation: RRID:Addgene_157922 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: wrmScarlet1-10
Vector Backbone Description: Backbone Size:6682; Vector Backbone:pRS423; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33693628
Proper citation: RRID:Addgene_158584 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3-tNCS-ePTS1
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158601 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO5 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
Proper citation: RRID:Addgene_14840 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScEap1p_91-150
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148710 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO5 promoter TATA-A1T6
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
Proper citation: RRID:Addgene_14846 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO5 promoter TATA-C2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by
homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start
codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb
hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start
codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation,
the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon
of ACT1 in the pJRL2 integrating vector.
Proper citation: RRID:Addgene_14844 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter TATA-A1T6
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
The GAL1 promoter was amplified from genomic DNA.
Proper citation: RRID:Addgene_14849 Copy
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