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Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Proper citation: RRID:Addgene_10747 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Proper citation: RRID:Addgene_10746 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10866658
Proper citation: RRID:Addgene_10753 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10866658
Proper citation: RRID:Addgene_10755 Copy
Species: Homo sapiens
Genetic Insert: VOPP1
Vector Backbone Description: Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30404791
Proper citation: RRID:Addgene_107507 Copy
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30404791
Proper citation: RRID:Addgene_107505 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11707428
Proper citation: RRID:Addgene_10760 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10767 Copy
Species: Danio rerio
Genetic Insert: U6a promoter
Vector Backbone Description: Backbone Size:2651; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107599 Copy
Genetic Insert: H2B-APEX2
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pDEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478324
Proper citation: RRID:Addgene_107597 Copy
Species: Homo sapiens
Genetic Insert: AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to insert a CAGGS-mTagRFPT-CAAX at the AAVS1 safe harbor locus in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After insertion of the reporter using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_107580 Copy
Genetic Insert: pCDNA3.1hygro-BamHI-CLuc1433-NotI
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29535383
Comments: To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.
Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.
Proper citation: RRID:Addgene_107611 Copy
Genetic Insert: NLucYAP15
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29535383
Comments: To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.
Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.
Proper citation: RRID:Addgene_107610 Copy
Species: Rattus norvegicus
Genetic Insert: VGF
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107575 Copy
Species: Mus musculus
Genetic Insert: Sp2
Vector Backbone Description: Backbone Marker:Guntram Suske (Addgene plasmid # 24544); Backbone Size:3996; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The plasmid is related to the Addgene plasmids pN3-Sp1FL (#24543), pN3-Sp3FL (#24541) and pN3-Control (#24544)
Proper citation: RRID:Addgene_107718 Copy
Vector Backbone Description: Backbone Marker:Guntram Suske (Addgene plasmid # 24544); Backbone Size:3996; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29381691
Comments: pN3-3xFlag is a derivative of the pN3-Control vector (Addgene 24544)
Proper citation: RRID:Addgene_107717 Copy
Species: dicot
Genetic Insert: Cas13a
Vector Backbone Description: Vector Backbone:pMOD_A; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28976959
Comments: This is a MOD_A plasmid compatible with the Cermek et al. toolkit.
https://www.addgene.org/browse/article/28189956/
Proper citation: RRID:Addgene_107691 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29379139
Comments: LOV2 domain starts with LAAA reported to stabilize Jα helix docking (Strickland et al. PMID: 22388287) and mutations I427V and H519L resulting in faster dark state recovery.
This LOV2 domain has the following mutations: I427V, H519L. The I427V mutation increases the dark state reversion rate of the LOV2 domain. Zdk1 binds efficiently to this LOV2 domain.
Proper citation: RRID:Addgene_107690 Copy
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29904106
Proper citation: RRID:Addgene_107720 Copy
Species: Rattus norvegicus
Genetic Insert: dLbCpf1-BE
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Comments: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)
Proper citation: RRID:Addgene_107685 Copy
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