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Species: Arabidopsis thaliana
Genetic Insert: AtUBC9
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104674 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC6
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104671 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC5
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104670 Copy
Species: Arabidopsis thaliana
Genetic Insert: MIEL1
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104713 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: Level 0 cloning vector for c terminal tag, clone insert without STOP codon BpiI
Proper citation: RRID:Addgene_104642 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: Level 0 cloning vector, clone insert BpiI
Proper citation: RRID:Addgene_104641 Copy
Species: Synthetic
Genetic Insert: N terminal HIS-Tag
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2071; Vector Backbone:pAGM3931; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone together with GOI to Level 1 cloning vector
Proper citation: RRID:Addgene_104658 Copy
Species: Synthetic
Genetic Insert: N terminal MBP-Tag
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29194629
Comments: release insert with BsaI and clone together with GOI to Level 1 cloning vector
Proper citation: RRID:Addgene_104656 Copy
Vector Backbone Description: Vector Backbone:pENTR top; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29087011
Comments: Alternative name of plasmid: pAH595
Proper citation: RRID:Addgene_104784 Copy
Vector Backbone Description: Vector Backbone:pENTR top; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29087011
Comments: Alternative name of plasmid: pNB184
Proper citation: RRID:Addgene_104786 Copy
Genetic Insert: exo, beta, gam, sgRNA-FRT
Vector Backbone Description: Backbone Marker:Prather lab; Backbone Size:6959; Vector Backbone:pKDsgRNA-ack; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29884781
Comments: Must be used in combination with plasmid pCas9-CR4 (Addgene plasmid # 62655) to express Cas9.
Proper citation: RRID:Addgene_113398 Copy
Genetic Insert: wbbL
Vector Backbone Description: Vector Backbone:pEXT21; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10574995
Comments: Please Note: The plasmid contains two wbbL inserts, which is not known to affect plasmid function.
Proper citation: RRID:Addgene_113662 Copy
Genetic Insert: g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
Vector Backbone Description: Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30760722
Proper citation: RRID:Addgene_115484 Copy
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10759496
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11572 Copy
Species: Arabidopsis thaliana
Genetic Insert: pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_115995 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116013 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116016 Copy
Species: Arabidopsis thaliana
Genetic Insert: pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116010 Copy
Species: Arabidopsis thaliana
Genetic Insert: pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116012 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116006 Copy
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