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Vector Backbone Description: Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Proper citation: RRID:Addgene_107682 Copy
Species: Homo sapiens
Genetic Insert: MAPRE3 gRNA #1 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107729 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1 gRNA #1 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107726 Copy
Species: mycobacterial
Genetic Insert: Che9C RecT
Vector Backbone Description: Backbone Marker:Sabine Ehrt and Dirk Schnappinger; Backbone Size:8026; Vector Backbone:pUV15tet-O; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25779316
Comments: Induced with anhydrotetracycline, the plasmid can be used to introduce single base pair changes in the chromosomes of M. smegmatis and M. tuberculosis.
For original description of methodology, see:
van Kessel, J.C. & Hatfull, G.F. Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets.
Mol Microbiol 67, 1094-1107 (2008).
Proper citation: RRID:Addgene_107770 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10775 Copy
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid was previously available as AV-9-PV4365 from the Penn Vector Core. GCaMP6s was created as part of the GENIE project at Janelia Research Campus.
Proper citation: RRID:Addgene_107790 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:3500; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29551490
Proper citation: RRID:Addgene_107707 Copy
Species: Homo sapiens
Genetic Insert: MAPRE3 gRNA #3 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107731 Copy
Species: Homo sapiens
Genetic Insert: MAPRE3 gRNA #2 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107730 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3988; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29379139
Proper citation: RRID:Addgene_107696 Copy
Genetic Insert: Cre, dTomato
Vector Backbone Description: Backbone Marker:Jonathan Ting; Backbone Size:7022; Vector Backbone:pAAV-hSyn1-DIO-SSFO-EYFP-P2A-nlsdTomato-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This plasmid can be used to express codon-optimized Cre and, separately, the red fluorescent dTomato. It is useful for studies aiming to fluorescently label cells that have undergone Cre-recombination of a Lox-containing DNA sequence.
Proper citation: RRID:Addgene_107738 Copy
Species: Mus musculus
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:8650; Vector Backbone:pCDH-EF1mcs-PGKpuro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28794185
Proper citation: RRID:Addgene_107735 Copy
Genetic Insert: Ag43
Vector Backbone Description: Backbone Marker:iGEM BioBricks; Backbone Size:4549; Vector Backbone:BBa_I0500-pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29555779
Comments: Ag43 contains a G981S variant compared to the NCBI reference (WP_000820410.1); plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_107743 Copy
Genetic Insert: Ag43
Vector Backbone Description: Backbone Marker:Andreas Moeglich Lab; Backbone Size:7293; Vector Backbone:pDawn; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29555779
Comments: Ag43 contains a G981S variant compared to the NCBI reference (WP_000820410.1); plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_107742 Copy
Species: Synthetic
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pAL; Vector Types:Worm Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29943731
Proper citation: RRID:Addgene_107745 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10786 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See author's map.
Proper citation: RRID:Addgene_10791 Copy
Species: Synthetic
Genetic Insert: Synthetic splicing reporter
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107859 Copy
Species: Homo sapiens
Genetic Insert: PKC epsilon RNAi
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15024053
Comments: Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGAT CGAGCTGGCT GTCTTTTTCA AGAGAAAAGA CAGCCAGCTC GATCTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGATCGA GCTGGCTGTC TTTTCTCTTG AAAAAGACAG CCAGCTCGAT CGGG-3'.
Proper citation: RRID:Addgene_10798 Copy
Species: Homo sapiens
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11964154
Comments: Constitutively active. Myr sequence from p60src.
Proper citation: RRID:Addgene_10797 Copy
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