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pUG-UBC9
 
Resource Report
Resource Website 		RRID:Addgene_104674 AtUBC9 Arabidopsis thaliana Spectinomycin PMID:29194629 release insert with BsaI and clone to Level 1 cloning vector Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin BpiI/BsaI sites removed by point-mutation 2026-08-15 01:00:44 0
pUG-UBC6
 
Resource Report
Resource Website 		RRID:Addgene_104671 AtUBC6 Arabidopsis thaliana Spectinomycin PMID:29194629 release insert with BsaI and clone to Level 1 cloning vector Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin BpiI/BsaI sites removed by point-mutation 2026-08-15 01:00:44 0
pUG-UBC5
 
Resource Report
Resource Website 		RRID:Addgene_104670 AtUBC5 Arabidopsis thaliana Spectinomycin PMID:29194629 release insert with BsaI and clone to Level 1 cloning vector Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin BpiI/BsaI sites removed by point-mutation 2026-08-15 01:00:44 0
pUG-131
 
Resource Report
Resource Website 		RRID:Addgene_104713 MIEL1 Arabidopsis thaliana Spectinomycin PMID:29194629 release insert with BsaI and clone to Level 1 cloning vector Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin BpiI/BsaI sites removed by point-mutation 2026-08-15 01:00:45 0
pUG-1287
 
Resource Report
Resource Website 		RRID:Addgene_104642 Spectinomycin PMID:29194629 Level 0 cloning vector for c terminal tag, clone insert without STOP codon BpiI Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:00:46 0
pUG-41308
 
Resource Report
Resource Website 		RRID:Addgene_104641 Spectinomycin PMID:29194629 Level 0 cloning vector, clone insert BpiI Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:00:44 0
pAGT861
 
Resource Report
Resource Website 		RRID:Addgene_104658 N terminal HIS-Tag Synthetic Spectinomycin PMID:29194629 release insert with BsaI and clone together with GOI to Level 1 cloning vector Backbone Marker:self-made; Backbone Size:2071; Vector Backbone:pAGM3931; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:00:44 0
pUG-225
 
Resource Report
Resource Website 		RRID:Addgene_104656 N terminal MBP-Tag Synthetic Spectinomycin PMID:29194629 release insert with BsaI and clone together with GOI to Level 1 cloning vector Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:00:44 0
pSC10
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_104784 Spectinomycin PMID:29087011 Alternative name of plasmid: pAH595 Vector Backbone:pENTR top; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:00:45 1
pSC12
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_104786 Spectinomycin PMID:29087011 Alternative name of plasmid: pNB184 Vector Backbone:pENTR top; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:00:48 1
pKDsgRNA-FRT
 
Resource Report
Resource Website 		RRID:Addgene_113398 exo, beta, gam, sgRNA-FRT Spectinomycin PMID:29884781 Must be used in combination with plasmid pCas9-CR4 (Addgene plasmid # 62655) to express Cas9. Backbone Marker:Prather lab; Backbone Size:6959; Vector Backbone:pKDsgRNA-ack; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:11 0
pMF19
 
Resource Report
Resource Website 		RRID:Addgene_113662 wbbL Spectinomycin PMID:10574995 Please Note: The plasmid contains two wbbL inserts, which is not known to affect plasmid function. Vector Backbone:pEXT21; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:14 0
5aa SunTag VP64 AP3
 
Resource Report
Resource Website 		RRID:Addgene_115484 g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS Spectinomycin PMID:30760722 Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:35 0
pMN19
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_11572 CaMV 35S enhancers Spectinomycin PMID:10759496 Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article. Kanamycin gene for plant selection on MS media. Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l). The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration. Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:36 1
pSW646 - pML1>>
 
Resource Report
Resource Website 		RRID:Addgene_115995 pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL47 - pSCR>>
 
Resource Report
Resource Website 		RRID:Addgene_116013 pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL94 - pOp4:VND7
 
Resource Report
Resource Website 		RRID:Addgene_116016 pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL44 - pVND7>>
 
Resource Report
Resource Website 		RRID:Addgene_116010 pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pVL46 - pWOX4>>
 
Resource Report
Resource Website 		RRID:Addgene_116012 pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0
pSW554 - pOp5:SP-mVenus-HDEL
 
Resource Report
Resource Website 		RRID:Addgene_116006 pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR Arabidopsis thaliana Spectinomycin PMID:30026289 Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:39 0

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