Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pGRG36 Resource Report Resource Website 1+ mentions |
RRID:Addgene_16666 | mTn7::MCS | Ampicillin | PMID:16646962 | Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101. | Backbone Size:12549; Vector Backbone:pGRG36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:37 | 7 | ||
|
pGRG25 Resource Report Resource Website 1+ mentions |
RRID:Addgene_16665 | mTn7::MCS | Ampicillin | PMID:16646962 | Grow at 32'C. Same as pGRG36 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101. | Backbone Size:12546; Vector Backbone:pGRG25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:36 | 2 | ||
|
Venus-BimEL(Noxa)-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166761 | BimEL(Mus musculus)-(Noxa BH3) | Homo sapiens | Kanamycin | PMID:35442739 | BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the β-barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | BH3 region removed and inserted BH3 of human Noxa protein | 2026-08-15 01:09:38 | 1 |
|
pETDuet-LahT150 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166785 | LahT150 | Lachnospiraceae bacterium C6A11 | Ampicillin | PMID:30638446 | Backbone Marker:Addgene; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 1 | ||
|
pETcon_SARS-CoV-2_RBD Resource Report Resource Website 1+ mentions |
RRID:Addgene_166782 | Spike receptor binding domain (RBD) from SARS-CoV-2 | Ampicillin | PMID:32841599 | Backbone Marker:Andrew Scharenberg; Vector Backbone:pETcon(-) (Plasmid #41522); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 6 | |||
|
Venus-BimL-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166734 | BimL | Mus musculus | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VBimL" (Venus fused to the N-terminus of the BH3-only protein, BimL) in live cells. There is a 3 amino acid flexible linker between Venus and BimL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
|
pLSB-NAT Resource Report Resource Website 1+ mentions |
RRID:Addgene_166698 | tRNA promoter : sgRNA cassette | Schizosaccharomyces pombe | Ampicillin | PMID:33313420 | The following acknowledgement must be included in all research publications and other outputs: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”. The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”. | Backbone Size:4988; Vector Backbone:pLSB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:37 | 2 | |
|
Venus-Bik-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166737 | Bik | Homo sapiens | Kanamycin | PMID:35442739 | Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
|
Venus-tBid-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166736 | tBid | Mus musculus | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VtBid" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. There is a 3 amino acid flexible linker between Venus and tBid proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
|
Venus-BimEL-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166735 | BimEL | Mus musculus | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
|
Venus-Puma-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166739 | Puma | Homo sapiens | Kanamycin | PMID:35442739 | Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 2 | |
|
pAM-DCA-muGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_166727 | muGFP | synthetic/engineered | Ampicillin | PMID:29330459 | Backbone Size:5405; Vector Backbone:pAM-DCA; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:37 | 1 | ||
|
mCerulean3-Mcl-1-pLVX Resource Report Resource Website 1+ mentions |
RRID:Addgene_166752 | Mcl-1 | Homo sapiens | Ampicillin | PMID:35442739 | 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins. | Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 1 | |
|
mCerulean3-Bcl-W-FUGW Resource Report Resource Website 1+ mentions |
RRID:Addgene_166751 | Bcl-W | Mus musculus | Ampicillin | PMID:35442739 | 3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W. | Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 1 | |
|
His6-TEV-Venus-pBluescript Resource Report Resource Website 1+ mentions |
RRID:Addgene_166756 | Venus | Synthetic | Ampicillin | PMID:35442739 | Plasmid can be used to express monomeric Venus fluorophore in bacteria. We used E.coli BL21-AI (Arabinose induction) for protein production, induced 5h at 30oC. Purify protein via Nickel column chromatography. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 1 | |
|
Venus-Bad-4E-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166740 | Bad-4E | Homo sapiens | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VBad-4E" (Venus fused to the N-terminus of the BH3-only protein, Bad-4E) in live cells. There is a flexible, 7 amino acid linker between Venus and Bad-4E proteins. Full length Bad protein with BH3 region hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: Y110E, L114E, M117E, F122E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 4 hydrophobic residues in the BH3 region mutated to glutamic acid: Y110E, L114E, M117E, F122E. | 2026-08-15 01:09:37 | 1 |
|
XE100 Pertussis toxin A protomer Resource Report Resource Website 1+ mentions |
RRID:Addgene_16678 | Pertussis toxin A protomer | Bordetella pertussis | Ampicillin | PMID:9389482 | Insert: Linearize with Not1 and transcribe with SP6. Ref: Slusarski et al., Nature 390:410-413, 1997. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:38 | 2 | |
|
Venus-tBid-4E-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166743 | tBid-4E | Mus musculus | Kanamycin | PMID:35442739 | Transfection of this plasmid will express "VtBid-4E" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E). BH3, Mutations: I27E, L31E, I34E, M38E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 4 hydrophobic residues in the BH3 region mutated to glutamic acid: I27E, L31E, I34E, M38E. | 2026-08-15 01:09:37 | 1 |
|
Venus-BimEL-4E-pEGFP-C1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166742 | BimEL-4E | Mus musculus | Kanamycin | PMID:30860026 | Transfection of this plasmid will express "VBimEL-4E" (Venus fused to the N-terminus of the BH3-only protein, BimEL-4E) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL-4E proteins. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: I146E, L150E, I153E, F157E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 4 hydrophobic residues in the BH3 region mutated to glutamic acid: I146E, L150E, I153E, F157E. | 2026-08-15 01:09:37 | 1 |
|
mCherry-Sec23A Resource Report Resource Website 1+ mentions |
RRID:Addgene_166894 | SEC23A | Homo sapiens | Kanamycin | PMID:33852913 | Vector Backbone:mCherry-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:39 | 2 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.