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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGRG36
 
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RRID:Addgene_16666 mTn7::MCS Ampicillin PMID:16646962 Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101. Backbone Size:12549; Vector Backbone:pGRG36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:37 7
pGRG25
 
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RRID:Addgene_16665 mTn7::MCS Ampicillin PMID:16646962 Grow at 32'C. Same as pGRG36 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101. Backbone Size:12546; Vector Backbone:pGRG25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:36 2
Venus-BimEL(Noxa)-pEGFP-C1
 
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RRID:Addgene_166761 BimEL(Mus musculus)-(Noxa BH3) Homo sapiens Kanamycin PMID:35442739 BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the β-barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin BH3 region removed and inserted BH3 of human Noxa protein 2026-08-15 01:09:38 1
pETDuet-LahT150
 
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RRID:Addgene_166785 LahT150 Lachnospiraceae bacterium C6A11 Ampicillin PMID:30638446 Backbone Marker:Addgene; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:38 1
pETcon_SARS-CoV-2_RBD
 
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RRID:Addgene_166782 Spike receptor binding domain (RBD) from SARS-CoV-2 Ampicillin PMID:32841599 Backbone Marker:Andrew Scharenberg; Vector Backbone:pETcon(-) (Plasmid #41522); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:38 6
Venus-BimL-pEGFP-C1
 
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RRID:Addgene_166734 BimL Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VBimL" (Venus fused to the N-terminus of the BH3-only protein, BimL) in live cells. There is a 3 amino acid flexible linker between Venus and BimL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
pLSB-NAT
 
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RRID:Addgene_166698 tRNA promoter : sgRNA cassette Schizosaccharomyces pombe Ampicillin PMID:33313420 The following acknowledgement must be included in all research publications and other outputs: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”. The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”. Backbone Size:4988; Vector Backbone:pLSB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:37 2
Venus-Bik-pEGFP-C1
 
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RRID:Addgene_166737 Bik Homo sapiens Kanamycin PMID:35442739 Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
Venus-tBid-pEGFP-C1
 
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RRID:Addgene_166736 tBid Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VtBid" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. There is a 3 amino acid flexible linker between Venus and tBid proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
Venus-BimEL-pEGFP-C1
 
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RRID:Addgene_166735 BimEL Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 1
Venus-Puma-pEGFP-C1
 
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RRID:Addgene_166739 Puma Homo sapiens Kanamycin PMID:35442739 Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:37 2
pAM-DCA-muGFP
 
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RRID:Addgene_166727 muGFP synthetic/engineered Ampicillin PMID:29330459 Backbone Size:5405; Vector Backbone:pAM-DCA; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:09:37 1
mCerulean3-Mcl-1-pLVX
 
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RRID:Addgene_166752 Mcl-1 Homo sapiens Ampicillin PMID:35442739 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins. Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:38 1
mCerulean3-Bcl-W-FUGW
 
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RRID:Addgene_166751 Bcl-W Mus musculus Ampicillin PMID:35442739 3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W. Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:38 1
His6-TEV-Venus-pBluescript
 
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RRID:Addgene_166756 Venus Synthetic Ampicillin PMID:35442739 Plasmid can be used to express monomeric Venus fluorophore in bacteria. We used E.coli BL21-AI (Arabinose induction) for protein production, induced 5h at 30oC. Purify protein via Nickel column chromatography. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:38 1
Venus-Bad-4E-pEGFP-C1
 
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RRID:Addgene_166740 Bad-4E Homo sapiens Kanamycin PMID:30860026 Transfection of this plasmid will express "VBad-4E" (Venus fused to the N-terminus of the BH3-only protein, Bad-4E) in live cells. There is a flexible, 7 amino acid linker between Venus and Bad-4E proteins. Full length Bad protein with BH3 region hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: Y110E, L114E, M117E, F122E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 4 hydrophobic residues in the BH3 region mutated to glutamic acid: Y110E, L114E, M117E, F122E. 2026-08-15 01:09:37 1
XE100 Pertussis toxin A protomer
 
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RRID:Addgene_16678 Pertussis toxin A protomer Bordetella pertussis Ampicillin PMID:9389482 Insert: Linearize with Not1 and transcribe with SP6. Ref: Slusarski et al., Nature 390:410-413, 1997. Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:38 2
Venus-tBid-4E-pEGFP-C1
 
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RRID:Addgene_166743 tBid-4E Mus musculus Kanamycin PMID:35442739 Transfection of this plasmid will express "VtBid-4E" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E). BH3, Mutations: I27E, L31E, I34E, M38E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 4 hydrophobic residues in the BH3 region mutated to glutamic acid: I27E, L31E, I34E, M38E. 2026-08-15 01:09:37 1
Venus-BimEL-4E-pEGFP-C1
 
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RRID:Addgene_166742 BimEL-4E Mus musculus Kanamycin PMID:30860026 Transfection of this plasmid will express "VBimEL-4E" (Venus fused to the N-terminus of the BH3-only protein, BimEL-4E) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL-4E proteins. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: I146E, L150E, I153E, F157E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 4 hydrophobic residues in the BH3 region mutated to glutamic acid: I146E, L150E, I153E, F157E. 2026-08-15 01:09:37 1
mCherry-Sec23A
 
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RRID:Addgene_166894 SEC23A Homo sapiens Kanamycin PMID:33852913 Vector Backbone:mCherry-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:39 2

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