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Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter TATA-A1T6
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
The GAL1 promoter was amplified from genomic DNA.
Proper citation: RRID:Addgene_14850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724390.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148973 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724395.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724390.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148976 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724395.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148979 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF8 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724396.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64521; Backbone Size:3833; Vector Backbone:pRG203MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154819 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64535; Backbone Size:5193; Vector Backbone:pRG205MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154815 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155043 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: QFGal4-switch
Vector Backbone Description: Backbone Size:7424; Vector Backbone:pT2-Ip2GI; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697972
Comments: Vector backbone derived from ubi:zebrabow (Plasmid #118219). mScarletNLS was codon-optimized for zebrafish by Tod Thiele (UTSC). ubi:QFGal4-switch can be used for Cre-dependent QFGal4 expression in lineage tracing experiments
Proper citation: RRID:Addgene_155125 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35 NM
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pAED4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495929
Comments: Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35.
Lindquist plasmid # 8635.
Proper citation: RRID:Addgene_15602 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35 NM
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pAED4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495929
Comments: Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35.
Lindquist plasmid # 8634.
Proper citation: RRID:Addgene_15601 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNF5
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 7151
Proper citation: RRID:Addgene_15597 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PUF2
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 8030
Proper citation: RRID:Addgene_15595 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CYC8
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 8029
Proper citation: RRID:Addgene_15594 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGPD1 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139489 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pCTT1 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139488 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pALD3 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1 24xMS2sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Depositor noted that MS2 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing.
Proper citation: RRID:Addgene_139493 Copy
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