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Species: Homo sapiens
Genetic Insert: SEC23A
Vector Backbone Description: Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166893 Copy
Species: Homo sapiens
Genetic Insert: SEC23A
Vector Backbone Description: Vector Backbone:Halo-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166892 Copy
Species: Mus musculus
Genetic Insert: VAMP2
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33658493
Proper citation: RRID:Addgene_166890 Copy
Species: Adeno-associated virus
Genetic Insert: AAV2 rep/AAV11 cap
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pSP72; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15567432
Comments: Adenovirus VA, E4, and E2a genes are also present on the backbone.
Proper citation: RRID:Addgene_166888 Copy
Vector Backbone Description: Backbone Marker:Rock Lab; Backbone Size:7670; Vector Backbone:plRL2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34297925
Proper citation: RRID:Addgene_166886 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6D; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.
Proper citation: RRID:Addgene_166835 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector containing NLRP1 (no stop codon)
Proper citation: RRID:Addgene_166823 Copy
Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428410
Comments: This encodes a secreted
antagonist of Xwnt-8.
The entire open reading frame of sizzled was amplified by low-cycle number PCR using Pfu polymerase and cloned as a
BamHI-XhoI fragment into pCS2+ .
Proper citation: RRID:Addgene_16688 Copy
Species: Xenopus laevis
Genetic Insert: APC
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9015311
Comments: Insert: Full length Xenopus APC with an amino terminal c-myc epitope in the vector pCS2+MT.
Insert size: ~8.5kb. NcoI site introduced at 5' end. Linearize with Not1 and transcribe with SP6.
Proper citation: RRID:Addgene_16686 Copy
Species: Homo sapiens
Genetic Insert: Dkk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11448771
Comments: This is wild-type, full-length Dickkopf-1, Flag tagged.
Proper citation: RRID:Addgene_16690 Copy
Species: Synthetic
Genetic Insert: Toehold Switch - Block G - Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166932 Copy
Species: Synthetic
Genetic Insert: Green Fluorescent Protein - Block F- Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:Twist Bioscience; Backbone Size:2221; Vector Backbone:pTwist Amp High Copy; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166931 Copy
Species: Synthetic
Genetic Insert: STOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166930 Copy
Species: Synthetic
Genetic Insert: PTAC promoter - Block H- Flanked by BsaI restriction sites.
Vector Backbone Description: Backbone Marker:GeneArt; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166936 Copy
Species: Synthetic
Genetic Insert: pT181 dual control activator (DC) - Flanked by BsaI restriction sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166935 Copy
Species: Synthetic
Genetic Insert: Star sense AD1.S6 - Block G- Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166934 Copy
Species: Synthetic
Genetic Insert: sRNA ToeHold - Block C- Flanked by BsaI sites
Vector Backbone Description: Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166925 Copy
Species: Synthetic
Genetic Insert: PTAC promoter - Block B- Flanked by BsaI restriction sites.
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166924 Copy
Species: Synthetic
Genetic Insert: Star Antisense AD1.A5- Block C- Flanked by BsaI restriction sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166926 Copy
Species: Homo sapiens
Genetic Insert: Sialyl Transferase N-terminus
Vector Backbone Description: Vector Backbone:EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18555781
Proper citation: RRID:Addgene_166943 Copy
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