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Genetic Insert: RAJ31 system (no terminator for the transRNA)
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4860; Vector Backbone:pSTC1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39257 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GGSGGS-nsp14
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Proper citation: RRID:Addgene_169161 Copy
Species: SARS-CoV-2
Genetic Insert: nsp10
Vector Backbone Description: Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Comments: Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells.
Proper citation: RRID:Addgene_169158 Copy
Species: Synthetic
Genetic Insert: protein G - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169105 Copy
Species: Synthetic
Genetic Insert: protein G - mNeonGreen - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169106 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Proper citation: RRID:Addgene_169243 Copy
Species: Escherichia coli
Genetic Insert: rhaRS
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the 2bp deletion in rTBC0541 (6796bp) as compared to the depositor's sequence does not affect plasmid function.
Proper citation: RRID:Addgene_169244 Copy
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the P10S mutation in the BLMT family bleomycin binding protein does not affect overall plasmid function.
Proper citation: RRID:Addgene_169245 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp12
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169188 Copy
Species: SARS-CoV-2
Genetic Insert: 14His-SUMO-nsp7
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169186 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GSGSGS-nsp16
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198328
Proper citation: RRID:Addgene_169191 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878)
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198325
Proper citation: RRID:Addgene_169193 Copy
Species: Bacillus circulans
Genetic Insert: Chitinase A1 Chitin Binding Domain
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437438v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_169211 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169409 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169411 Copy
Genetic Insert: mWasabi
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169412 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169410 Copy
Species: Mus musculus
Genetic Insert: HaloTAG mGFAP (R236H)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_169475 Copy
Vector Backbone Description: Backbone Size:6472; Vector Backbone:pPS04; Vector Types:Allelic exchange vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_169472 Copy
Species: Synthetic
Genetic Insert: anti-caffeine nanobody
Vector Backbone Description: Backbone Size:4900; Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33552855
Proper citation: RRID:Addgene_169685 Copy
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