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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pEMB018 / LRRK2_BC_1309_2232
 
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RRID:Addgene_40526 LRRK2 Homo sapiens Ampicillin Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin WT; aa1309_2232 2026-08-15 01:14:37 0
pBACgus4x/Parkin/RocCOR LRRK2 1867-2176
 
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RRID:Addgene_40402 LRRK2 Homo sapiens Ampicillin Co-expresses Parkin Backbone Marker:Novagen; Vector Backbone:pBACgus4x/Parkin; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin WT aa1867-2176 2026-08-15 01:14:36 0
pEMB018 / LRRK2_BC_1309_2527_M2397T
 
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RRID:Addgene_40523 LRRK2 Homo sapiens Ampicillin Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin aa1309_2527; M2397T 2026-08-15 01:14:37 0
pEMB018 / LRRK2_BC_1520_2210
 
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RRID:Addgene_40522 LRRK2 Homo sapiens Ampicillin Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin WT; aa1520_2210 2026-08-15 01:14:37 0
pEMB018 / LRRK2_BC_1520_2187
 
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RRID:Addgene_40521 LRRK2 Homo sapiens Ampicillin Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin WT; aa1520_2187 2026-08-15 01:14:37 0
pEMB018 / LRRK2_BC_1309_2350
 
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RRID:Addgene_40529 LRRK2 Homo sapiens Ampicillin Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin WT; aa1309_2350 2026-08-15 01:14:37 0
pGEX4T-1_OCT4_T234A
 
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RRID:Addgene_40637 POU5F1 Homo sapiens Ampicillin PMID:22474382 Backbone Marker:GE-healthcare; Backbone Size:6237; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T234A 2026-08-15 01:14:38 0
pGEX4T-1_OCT4_T234E
 
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RRID:Addgene_40636 POU5F1 Homo sapiens Ampicillin PMID:22474382 Backbone Marker:GE-healthcare; Backbone Size:6237; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T234E 2026-08-15 01:14:38 0
pGEX4T-1_OCT4_T234A_S235A
 
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RRID:Addgene_40634 POU5F1 Homo sapiens Ampicillin PMID:22474382 Backbone Marker:Invitrogen; Backbone Size:10712; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin T234A_S235A 2026-08-15 01:14:38 0
pGEX4T-1_OCT4_S235A
 
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RRID:Addgene_40639 POU5F1 Homo sapiens Ampicillin PMID:22474382 Backbone Marker:GE-healthcare; Backbone Size:6237; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin S235A 2026-08-15 01:14:38 0
pCEP4_OCT4_T234A_S235A
 
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1+ mentions
RRID:Addgene_40630 POU5F1_S234A_T235A Homo sapiens Ampicillin PMID:22474382 Backbone Marker:Invitrogen; Backbone Size:10712; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S234A_T235A 2026-08-15 01:14:38 1
pRMH120
 
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1+ mentions
RRID:Addgene_40593 CRY2 Arabidopsis thaliana Ampicillin PMID:22922268 Backbone Size:5414; Vector Backbone:p414TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:37 1
hGFAP-Cre
 
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1+ mentions
RRID:Addgene_40591 Cre recombinase Homo sapiens Ampicillin PMID:11668683 This plasmid encodes a transgene to express the Cre recombinase in astrocytes of transgenic mice. A DNA fragment encoding the Cre recombinase was inserted into an expression cassette containing the gfa2 promoter (Besnard et al., 1991), a 2.2-kb 5' flanking region from the human GFAP (hGFAP) gene. The nuclear targeting signal from the SV40 large T antigen was inserted at the amino terminal end of the coding region, because it reportedly increases the efficiency of DNA excision by the recombinase (Gu et al., 1993). A heterologous intron and polyadenylation signal were provided by sequences from the mouse protamine 1 gene at the 3' end. The hGFAP-cre transgene was constructed by excising the lacZ coding region from pgfa2-lac2 (Brenner et al., 1994) by BamHI digestion, and replacing it by blunt end ligation with a nuclear-targeted Cre coding sequence. The latter was obtained as a 1.1-kb SalI-MluI fragment from plasmid pTZ19RCreNLS, which was kindly provided by Drs. Raul Torres and Klaus Rajewsky. Both junctions were verified by DNA sequencing. Backbone Marker:Michael Brenner (PMID: 7952266); Backbone Size:5500; Vector Backbone:pgfa2-lac2; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin Construct contains a nuclear-targeted Cre recombinase followed by a heterologous mouse intron and polyadenylation signal, all under the control of the gfa2 promoter 2026-08-15 01:14:37 8
FUGW-H1-Syndecan-1 shRNA (UTR)
 
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1+ mentions
RRID:Addgene_40623 Syndecan-1 shRNA(UTR) Mus musculus Ampicillin PMID:22936997 This shRNA plasmid was generated by inserting the hairpin oligonucleotides into the FUGW-H1 lentiviral construct. Backbone Size:10130; Vector Backbone:FUGW-H1; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:14:38 2
pXL010-Wnt dual (GFP-Fire) reporter
 
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1+ mentions
RRID:Addgene_40588 Wnt reporter sequences Homo sapiens Ampicillin PMID:22177620 This lentiviral Wnt reporter has 7 repeats of Tcf/Lef binding sites, which was cloned by Xiaojun (Lance) Lian. Methods for Plasmid construction, lentiviral production and infection of hESCs: The 7 TCF/LEF binding site sequences were obtained by direct PCR of plasmid M50 (Addgene plasmid 12456) and the GreenFire (GF) sequences were obtained by direct PCR of the pGreenFire1-mCMV Plasmid (System Bioscience TR010PA-1). These two sequences were cloned into pSicoR PGK puro (Addgene plasmid 11586) digested by Xba I (NEB) and Xho I (NEB). The constructed Wnt reporter plasmid was named 7TGFP and verified by sequencing. This 7TGFP vector was cotransfected with the helper plasmids psPAX2 and pMD2.G (Addgene plasmids 12260 and 12259) into HEK-293TN cells (System Biosciences) for virus production. Virus-containing medium was collected at 48 and 72 hours after transfection and used for infection of hESCs in the presence of 6μg/mL polybrene (Sigma). Transduced cells were cultured in mTeSR1 on Matrigel for three days and then clonally isolated in mTeSR1 with 1 μg/mL puromycin. Backbone Marker:addgene; Vector Backbone:pSicoR PGK Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:37 3
pcDNA3-HA-TCF1
 
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1+ mentions
RRID:Addgene_40620 TCF7 Homo sapiens Ampicillin PMID:22949634 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:38 4
PP-ASL
 
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RRID:Addgene_40749 Trypanosoma Brucei Adenylosuccinate Lyase Trypanosoma Brucei Ampicillin http://www.thesgc.org/structures/4EFC/ Tb427tmp.160.5560:MAC049-C01:C223709 Backbone Marker:SGC; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:38 0
pRMH124-VP16-CIB1
 
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RRID:Addgene_40615 VP16-CIB1 Arabidopsis thaliana Ampicillin PMID:22922268 Backbone Marker:Clontech; Backbone Size:7500; Vector Backbone:pGADT7rec; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:37 0
719-2µ-minHIS3
 
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RRID:Addgene_40610 HIS3 Saccharomyces cerevisiae Ampicillin PMID:24357599 Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6512; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Every codon of the coding sequence has been replaced with the isoaccepting codon decoded by the least abundant tRNA. 2026-08-15 01:14:37 0
pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
 
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RRID:Addgene_40731 Aurora B FRET-sensor Synthetic Ampicillin PMID:22801782 Backbone Marker:Clontech; Backbone Size:5125; Vector Backbone:pIRES-puro2b; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:38 0

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