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Species: Homo sapiens
Genetic Insert: MCAK-mCherry-eDHFR
Vector Backbone Description: Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904910
Proper citation: RRID:Addgene_176204 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17623 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17626 Copy
Species: Homo sapiens
Genetic Insert: spCas9 and polymerase exo domain fusion
Vector Backbone Description: Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35323942
Proper citation: RRID:Addgene_176236 Copy
Species: Homo sapiens
Genetic Insert: spCas9 and polymerase exo domain fusion
Vector Backbone Description: Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35323942
Proper citation: RRID:Addgene_176237 Copy
Species: Homo sapiens
Genetic Insert: JAZF1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176352 Copy
Species: Homo sapiens
Genetic Insert: TSC22D3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176355 Copy
Species: Homo sapiens
Genetic Insert: SOX15
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176356 Copy
Species: Homo sapiens
Genetic Insert: POU6F1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: SK-N-SH cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176359 Copy
Species: Homo sapiens
Genetic Insert: GRHL2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176351 Copy
Species: Homo sapiens
Genetic Insert: ZNF468
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176350 Copy
Species: Homo sapiens
Genetic Insert: ID3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176342 Copy
Species: Homo sapiens
Genetic Insert: CDX4
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176343 Copy
Species: Homo sapiens
Genetic Insert: NPAS1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176346 Copy
Species: Homo sapiens
Genetic Insert: CGGBP1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176345 Copy
Species: Homo sapiens
Genetic Insert: THAP12
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176348 Copy
Species: Homo sapiens
Genetic Insert: DNAJC1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176340 Copy
Species: Homo sapiens
Genetic Insert: ZNF79
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176371 Copy
Species: Homo sapiens
Genetic Insert: SOX2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176364 Copy
Species: Homo sapiens
Genetic Insert: OSR1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: SK-N-SH cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176363 Copy
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