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Species: Homo sapiens
Genetic Insert: Z3CH10
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: SK-N-SH cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176368 Copy
Species: Homo sapiens
Genetic Insert: POU5F1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176367 Copy
Species: Homo sapiens
Genetic Insert: NR2E3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: A549 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176369 Copy
Species: Homo sapiens
Genetic Insert: ZNF608
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176362 Copy
Species: Homo sapiens
Genetic Insert: ZNF608 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176380 Copy
Species: Homo sapiens
Genetic Insert: SOX2 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176382 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33891012
Comments: GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression.
Proper citation: RRID:Addgene_176462 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33891012
Comments: GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression.
Proper citation: RRID:Addgene_176465 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33891012
Comments: EYFP is internally inserted to replace the alternative skipping exon 40 of VPS13D (in-frame)
Proper citation: RRID:Addgene_176464 Copy
Species: Homo sapiens
Genetic Insert: EGFP-T2A-TFEB(human; delta30; amino acids 31-476)
Vector Backbone Description: Vector Backbone:pCamR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29507340
Proper citation: RRID:Addgene_176500 Copy
Species: Homo sapiens
Genetic Insert: HP1 beta
Vector Backbone Description: Backbone Marker:Zentgraf Lab; Backbone Size:0; Vector Backbone:pBCHGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12560555
Comments: See author's map for more information.
Proper citation: RRID:Addgene_17651 Copy
Species: Homo sapiens
Genetic Insert: RBBP7
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
Comments: SGC link: http://www.thesgc.org/structures/7M3X/.
Proper citation: RRID:Addgene_176411 Copy
Species: Homo sapiens
Genetic Insert: EGFP-T2A-TFEB(human; full-length; S3E mutation)-
Vector Backbone Description: Vector Backbone:pCamR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29507340
Proper citation: RRID:Addgene_176499 Copy
Species: Homo sapiens
Genetic Insert: HP1 alpha
Vector Backbone Description: Backbone Marker:Zentgraf Lab; Backbone Size:0; Vector Backbone:pBCHGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12560555
Comments: See author's map for more information.
Proper citation: RRID:Addgene_17652 Copy
Species: Homo sapiens
Genetic Insert: D50 lamin A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15750600
Proper citation: RRID:Addgene_17653 Copy
Species: Homo sapiens
Genetic Insert: Nurr1
Vector Backbone Description: Backbone Size:11000; Vector Backbone:Piggybac TetO - Blastocidin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34171286
Proper citation: RRID:Addgene_176485 Copy
Species: Homo sapiens
Genetic Insert: Lmx1A-T2A-Pitx3
Vector Backbone Description: Backbone Size:6585; Vector Backbone:Piggybac TetO - puromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34171286
Proper citation: RRID:Addgene_176484 Copy
Species: Homo sapiens
Genetic Insert: En1-t2a-FoxA2
Vector Backbone Description: Backbone Size:8621; Vector Backbone:Piggybac TetO - hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34171286
Proper citation: RRID:Addgene_176483 Copy
Species: Homo sapiens
Genetic Insert: Alix (360-702)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17277784
Comments: The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
Proper citation: RRID:Addgene_17639 Copy
Species: Homo sapiens
Genetic Insert: NR2E3 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176386 Copy
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