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Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Scarlet(rfp)-Link-TUB1
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179054 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-ARC35-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179055 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TDH3p-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179077 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AMN1 (left homology arm) - KanMX - AMN1 (right homology arm)
Vector Backbone Description: Backbone Marker:Harvard Medical School; Backbone Size:3607; Vector Backbone:pBY011; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35361876
Proper citation: RRID:Addgene_183099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mito-yHS1
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32265272
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159153 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mito-yHS1-M7A,H102A
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32265272
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159165 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4-transactivator
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169582 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Yeast MCP1
Vector Backbone Description: Backbone Size:6281; Vector Backbone:pCMV10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35357422
Proper citation: RRID:Addgene_184341 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
Vector Backbone Description: Backbone Size:5453; Vector Backbone:pPT24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35634362
Proper citation: RRID:Addgene_184339 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PhoCl-TsCC(B)
Vector Backbone Description: Vector Backbone:pfa6a::KAN; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34341589
Proper citation: RRID:Addgene_185906 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FRB-RGG-GFP-RGG-RGG
Vector Backbone Description: Vector Backbone:Yiplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34341589
Proper citation: RRID:Addgene_176400 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19723816
Proper citation: RRID:Addgene_185065 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SWI6
Vector Backbone Description: Vector Backbone:pOAD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35189816
Proper citation: RRID:Addgene_185134 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CRM1
Vector Backbone Description: Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35189816
Proper citation: RRID:Addgene_185133 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFA2
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15294903
Proper citation: RRID:Addgene_185075 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SWI6
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35189816
Proper citation: RRID:Addgene_185081 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7929578
Comments: Please note: Plasmid contains an additional 22 amino acids (LKDIYSYIPSTYTLHMKLLEKF) on the C-terminus of Nup1 compared to the NCBI reference sequence NP_014741.1. It is not known how this affect the plasmid's function.
Proper citation: RRID:Addgene_185431 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7929578
Proper citation: RRID:Addgene_185430 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP2
Vector Backbone Description: Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains a Q9R mutation in NUP2 compared to the NCBI reference sequence NP_013439.1. It is not known how this mutation affects plasmid function.
Proper citation: RRID:Addgene_185435 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP2
Vector Backbone Description: Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains a S137F mutation in Nup2 compared to the NCBI reference sequence NP_013439.1. It is not known how this mutation affects plasmid function.
Proper citation: RRID:Addgene_185428 Copy
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