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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCXLE-hOCT3/4 Resource Report Resource Website 50+ mentions |
RRID:Addgene_27076 | OCT3/4 | Homo sapiens | Ampicillin | PMID:21460823 | Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:18 | 52 | ||
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pGEX6-Smad3 EPSM A213S Resource Report Resource Website |
RRID:Addgene_27110 | Smad3 | Homo sapiens | Ampicillin | PMID:19917253 | A213 was mutated back to the wild type residue in EPSM construct | Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-6P; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | T179V S204A S208A S213A; A213S | Addgene | 2026-10-31 02:08:19 | 0 |
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pGV13 (CK2alpha K68M, CK2beta) Resource Report Resource Website |
RRID:Addgene_27094 | CK2beta | Homo sapiens | Ampicillin | PMID:21036246 | Backbone Marker:Clontech; Backbone Size:4358; Vector Backbone:pBI; Vector Types:Mammalian Expression, tetracycline-regulated expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:18 | 0 | ||
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pGV7 (CK2alpha' K69M, CK2beta) Resource Report Resource Website |
RRID:Addgene_27095 | CK2beta | Homo sapiens | Ampicillin | PMID:21036246 | Backbone Marker:Clontech; Backbone Size:4358; Vector Backbone:pBI; Vector Types:Mammalian Expression, tetracycline-regulated expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:18 | 0 | ||
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pGP18 (CK2alpha' K69M) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27090 | CK2alpha' K69M | Homo sapiens | Ampicillin | PMID:21036246 | Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K69M | Addgene | 2026-10-31 02:08:18 | 1 | |
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pKSJ155 Resource Report Resource Website |
RRID:Addgene_27126 | Colicin Ia + Ia immunity protein | E. coli | Ampicillin | PMID:11004207 | This plasmid contains the C-terminal channel forming domain of colicin Ia and the Ia immunity protein. The construct is also referred to as "CT-Medium or CT-M". The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other. | Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:19 | 0 | |
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pGMCS-0X-P38-PanBmGFP Resource Report Resource Website |
RRID:Addgene_27127 | PanB | Mycobacterium tuberculosis | Streptomycin | PMID:20711362 | Backbone Size:5400; Vector Backbone:pGMCS; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | Addgene | 2026-10-31 02:08:19 | 0 | ||
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eTC GFP ß-actin ΔZip Resource Report Resource Website 1+ mentions |
RRID:Addgene_27124 | beta-actin | Homo sapiens | Ampicillin | PMID:17030983 | Note: Addgene's sequencing results indicate the presence of several nucleotide inserts (starting at bp# 2459, 2501 and 2516) when compared to the sequence provided by the depositing laboratory. The depositing scientist confirmed that our sequencing result is correct and the inserts do not affect the function of the plasmid. | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | This construct lacks β-actin 3′UTR and therefore the zipcode sequence | Addgene | 2026-10-31 02:08:19 | 4 |
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pZW16 (CK2alpha') Resource Report Resource Website 1+ mentions |
RRID:Addgene_27087 | CK2alpha' | Homo sapiens | Ampicillin | PMID:21036246 | Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:18 | 3 | ||
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pZW12 (CK2beta) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27088 | CK2beta | Homo sapiens | Ampicillin | PMID:21036246 | Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:18 | 5 | ||
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pMS2-GFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_27121 | MS2 | bacteriophage MS2 | Ampicillin | PMID:12546792 | Under the control of the promoter of the large subunit of RNA polymerase II NB: There is a 14aa gap in the MS2 sequence starting at aa#67 when compared with GenBank ID: NP_040648.1 | Backbone Size:0; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:19 | 43 | |
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pAB46 (CK2beta) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27085 | CK2beta | Homo sapiens | Kanamycin | PMID:21036246 | Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:18 | 1 | ||
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pZW6 (CK2alpha) Resource Report Resource Website 10+ mentions |
RRID:Addgene_27086 | CK2alpha | Homo sapiens | Ampicillin | PMID:21036246 | Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:18 | 15 | ||
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pMODloxZeo3DAmp Resource Report Resource Website |
RRID:Addgene_27182 | lox-EM7p-Zeo-lox cassette flanked by Tn5 outer element | synthetic | Bleocin (Zeocin) | PMID:15784610 | Note that Bleomycin and Zeocin are the same antibiotic. | Backbone Marker:Epicentre; Backbone Size:2000; Vector Backbone:pMOD |
Addgene | 2026-10-31 02:08:19 | 0 | |
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CACNA1F_R (OZ508) Resource Report Resource Website |
RRID:Addgene_27183 | Zinc finger array targeting cacna1f | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene CACNA1F (see below). Please note that this plasmid does NOT contain the CACNA1F sequence. Users must order the complementary plasmid CACNA1F_L (OZ507) [Addgene plasmid 26957] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TAAGAAGAG-3' in a bacterial two hybrid assay, and resulted in 4.21 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:19 | 0 | |
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pBSDT-AII Resource Report Resource Website 1+ mentions |
RRID:Addgene_27179 | MC1 promoter diphtheria toxin-A fragment | synthetic | Ampicillin | PMID:15784610 | The MCS in the pBSDT-AII is extensibly modified by synthetic oligos to create multiple rare restriction sites | Backbone Marker:Stratagene; Backbone Size:2800; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:19 | 1 | |
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p3XFLAG-MKL2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27175 | MKL2 | Homo sapiens | Ampicillin | PMID:14565952 | Backbone Marker:Sigma; Backbone Size:0; Vector Backbone:p3XFLAG-CMV7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:19 | 3 | ||
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pCGN-ATF6 (1-373) Resource Report Resource Website 10+ mentions |
RRID:Addgene_27173 | ATF6 | Homo sapiens | Ampicillin | PMID:10856300 | This is the activated (nuclear) form of ATF6. Please note that mutation M13I in ATF6 was found during Addgene's quality control. The plasmid should function as described in the associated publication above. | Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Contains only aa 1-373 (please see depositor comments below) | Addgene | 2026-10-31 02:08:19 | 14 |
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pGMCZ-PrpoB Resource Report Resource Website |
RRID:Addgene_27073 | P-rpoB | M. smegmatis | Bleocin (Zeocin) | PMID:21075796 | Backbone Size:5390; Vector Backbone:pDE43-MCZ; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin) | Addgene | 2026-10-31 02:08:18 | 0 | ||
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pitrm1_L (OZ515) Resource Report Resource Website |
RRID:Addgene_27190 | Zinc finger array targeting pitrm1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene pitrm1 (see below). Please note that this plasmid does NOT contain the pitrm1 sequence. Users must order the complementary plasmid pitrm1_R (OZ516) [Addgene plasmid 27191] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGCAGGT-3' in a bacterial two hybrid assay, and resulted in 6.84 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:19 | 0 |
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