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Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector W-Y
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 25 of EMMA platform.
Proper citation: RRID:Addgene_100665 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector W-X
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 24 of EMMA platform.
Proper citation: RRID:Addgene_100666 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector T-V
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 19 and 22 of EMMA platform.
Proper citation: RRID:Addgene_100662 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector U-V
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 20 and 22 of EMMA platform.
Proper citation: RRID:Addgene_100663 Copy
Species: Homo sapiens
Genetic Insert: sgSTOP targeting TIMELESS (cloned using BbsI)
Vector Backbone Description: Vector Backbone:B52 plasmid expressing TIMELESS sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28890334
Proper citation: RRID:Addgene_100713 Copy
Species: Homo sapiens
Genetic Insert: Kozak-ATG-MLS
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Kozak-ATG-MLS (Mitochondrial Localization Sequence)
Proper citation: RRID:Addgene_100676 Copy
Genetic Insert: SV40-polyA
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: SV40-polyA terminator sequence.
Proper citation: RRID:Addgene_100678 Copy
Species: Homo sapiens
Genetic Insert: sgSTOP targeting PARP4 (cloned using BbsI)
Vector Backbone Description: Vector Backbone:B52 plasmid expressing PARP4 sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28890334
Proper citation: RRID:Addgene_100711 Copy
Species: Homo sapiens
Genetic Insert: sgSTOP targeting CHEK2 (cloned using BbsI)
Vector Backbone Description: Vector Backbone:B52 plasmid expressing CHEK2 sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28890334
Proper citation: RRID:Addgene_100712 Copy
Species: Rattus norvegicus
Genetic Insert: SaBE4
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28875174
Proper citation: RRID:Addgene_100805 Copy
Species: Rattus norvegicus
Genetic Insert: BE4-Gam
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28875174
Proper citation: RRID:Addgene_100806 Copy
Species: Rattus norvegicus
Genetic Insert: BE3-Gam
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28875174
Proper citation: RRID:Addgene_100807 Copy
Species: rabies virus
Genetic Insert: rabies virus CVS N2c strain phosphoprotein gene
Vector Backbone Description: Backbone Marker:Connie Cepko; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_100808 Copy
Species: rabies virus
Genetic Insert: rabies virus CVS N2c strain nucleoprotein gene
Vector Backbone Description: Backbone Marker:Connie Cepko; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_100801 Copy
Species: Rattus norvegicus
Genetic Insert: BE
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28875174
Proper citation: RRID:Addgene_100802 Copy
Species: Synthetic
Genetic Insert: CDA1-BE3
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28875174
Proper citation: RRID:Addgene_100804 Copy
Species: Synthetic
Genetic Insert: Lenti-Mito-Car-GECO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7067; Vector Backbone:pLenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29511336
Comments: sub-cloned from Addgene CMV-mito-CAR-GECO1 #46022
Proper citation: RRID:Addgene_100765 Copy
Genetic Insert: fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
Vector Backbone Description: Backbone Marker:Connie Cepko; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_100800 Copy
Species: Homo sapiens
Genetic Insert: GST-Nix-S212A
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4900; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use E. coli LB21 for expression
Proper citation: RRID:Addgene_100760 Copy
Species: Homo sapiens
Genetic Insert: GST-Nix-S212D
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4900; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use E. coli LB21 for expression
Proper citation: RRID:Addgene_100761 Copy
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