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Species: Rattus norvegicus
Genetic Insert: beta-arrestin2
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1 zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Proper citation: RRID:Addgene_36917 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3x mCherry
Vector Backbone Description: Backbone Size:9951; Vector Backbone:pSinRep5; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327349
Proper citation: RRID:Addgene_36914 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin1
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1 zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Proper citation: RRID:Addgene_36916 Copy
Species: HIV-I
Genetic Insert: fNL43-dE-EGFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Comments: Plasmid region from nt9236 to nt308 is derived from pNL43dSL1.
Proper citation: RRID:Addgene_36865 Copy
Genetic Insert: T (i.e., ATG, start codon as a first exon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Comments: This is the 'LNL' version of the plasmid. It contains loxP-Neo-loxP in the intron. The 'FLLFLNL' version (loxP-Neo-loxP) is also available from Addgene.
Proper citation: RRID:Addgene_36908 Copy
Species: Ciona intestinalis
Genetic Insert: fluorescent protein voltage sensor
Vector Backbone Description: Backbone Size:5694; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22958819
Comments: http://www.fluorogenetic-voltage-sensors.org/
This construct is composed of the S1-4 domain of the CiVSP voltage sensor. Super ecliptic pHluorin is inserted at position S249 in the CiVSP sequence.
Proper citation: RRID:Addgene_36855 Copy
Species: Ciona intestinalis
Genetic Insert: fluorescent protein voltage sensor
Vector Backbone Description: Backbone Size:5694; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22958819
Comments: http://www.fluorogenetic-voltage-sensors.org/
This construct is composed of the S1-4 domain of the CiVSP voltage sensor. Super ecliptic pHluorin is inserted at position A242 in the CiVSP sequence.
Proper citation: RRID:Addgene_36857 Copy
Species: Homo sapiens
Genetic Insert: SIRT6-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCCGGGAGGCCGCACTTG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36844 Copy
Vector Backbone Description: Backbone Size:7037; Vector Backbone:pCMMP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12972608
Comments: Sugden lab plasmid p3051
Proper citation: RRID:Addgene_36953 Copy
Genetic Insert: LMP1
Vector Backbone Description: Backbone Marker:Addgene plasmid #36953; Backbone Size:7037; Vector Backbone:pCMMP-MCS-IRES-eGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318153
Comments: Sugden lab plasmid p3455
Please note that the full plasmid sequence provided by the depositing laboratory and Addgene's sequencing results differ from GenBank entry YP_401722.1 by three polymorphisms: F106Y, I122L and M129I. These differences result from the particular EBV isolate. For more information please see the following article: https://www.ncbi.nlm.nih.gov/pubmed/7931160
Proper citation: RRID:Addgene_36955 Copy
Species: Homo sapiens
Genetic Insert: EglN2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6886; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36950 Copy
Species: Homo sapiens
Genetic Insert: EglN3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36951 Copy
Vector Backbone Description: Backbone Size:7037; Vector Backbone:pCMMP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18042799
Comments: Sugden lab plasmid p3048
Proper citation: RRID:Addgene_36952 Copy
Species: Homo sapiens
Genetic Insert: DNMT3B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15861382
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI restriction site (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.
The full-length cDNA for human DNMT3B2 was cloned into EcoRI and BamHI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen.
Proper citation: RRID:Addgene_36942 Copy
Species: Aequorea victoria
Genetic Insert: Venus (EYFP)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the laboratory of an HHMI investigator
Proper citation: RRID:Addgene_36948 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22249253
Proper citation: RRID:Addgene_37104 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22249253
Comments: Also known as PSM.7LP (Knudsen and Wang 1997 MCB 17:5771).
Proper citation: RRID:Addgene_37106 Copy
Species: Homo sapiens
Genetic Insert: PHLPP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454620
Comments: Please note that the insert in this plasmid was referred to as PHLPP1beta in the original publication (Brognard et al., Mol. Cell 2007) and the plasmid was first described in Warfel et al. 2011.
Proper citation: RRID:Addgene_37100 Copy
Species: Homo sapiens
Genetic Insert: Human SMN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16301532
Proper citation: RRID:Addgene_37057 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #37060; Vector Backbone:pHSS6; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Used to create strains to complement mutations using the NICS system (Mehr and Seifert, 1998; Mehr, 2000). Insert a functional copy of the gene ectopically at an unlinked chromosomal locus between the lctP and aspC genes under control of lac regulatory sequences, and linked to an Erythromycin-resistance cassette.
Proper citation: RRID:Addgene_37058 Copy
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