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Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24747889
Proper citation: RRID:Addgene_51628 Copy
Species: Mus musculus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23991227
Proper citation: RRID:Addgene_51595 Copy
Species: Mus musculus
Genetic Insert: shRNA1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24036351
Proper citation: RRID:Addgene_51597 Copy
Species: Mus musculus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23575848
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The Rem2 insert in this plasmid contains T13I and T15I mutations when compared to GenBank reference sequence NP_542764.2.
Proper citation: RRID:Addgene_51591 Copy
Species: Mus musculus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23575848
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The Rem2 insert in this plasmid contains T13I and T15I mutations when compared to GenBank reference sequence NP_542764.2.
Proper citation: RRID:Addgene_51593 Copy
Species: Homo sapiens
Genetic Insert: Pontin
Vector Backbone Description: Backbone Marker:Invitrogen/LIfe Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18358808
Proper citation: RRID:Addgene_51635 Copy
Species: Synthetic
Genetic Insert: Codon-optimized Cas9 D10A
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24491566
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) using the primers (Forward primer, 5’-AGTACTCCATTGGGCTCGccATCGGCACAAAC, and Reverse primer, 5’-CGAGCCCAATGGAGTACTTCTTGTCGGCTGC).
For the in vitro synthesis of CAS9 D10A mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA.
The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment.
The CAG promoter in this plasmid also allows it to be used as a CAS9-expression vector.
Proper citation: RRID:Addgene_51638 Copy
Species: Homo sapiens
Genetic Insert: TERT
Vector Backbone Description: Backbone Marker:Invitrogen/LIfe Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18358808
Comments: Addgene full plasmid sequencing noted a conserved E286D substitution in hTert.
Proper citation: RRID:Addgene_51637 Copy
Species: Mus musculus
Genetic Insert: Sema4D
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24036351
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The Sema4D insert in this plasmid does not contain amino acids 1-50 and contains a L745P mutation when compared to GenBank reference sequence NP_542764.2
Proper citation: RRID:Addgene_51599 Copy
Species: Homo sapiens
Genetic Insert: ADAM10
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 6
Proper citation: RRID:Addgene_51641 Copy
Species: Homo sapiens
Genetic Insert: ACVR1B
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 5
Proper citation: RRID:Addgene_51640 Copy
Species: Homo sapiens
Genetic Insert: CD34
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 13
Proper citation: RRID:Addgene_51647 Copy
Species: Homo sapiens
Genetic Insert: CD226
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 12
Proper citation: RRID:Addgene_51646 Copy
Species: Homo sapiens
Genetic Insert: APLP2
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 7
Proper citation: RRID:Addgene_51642 Copy
Species: Homo sapiens
Genetic Insert: GP5
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 24
Proper citation: RRID:Addgene_51645 Copy
Species: Homo sapiens
Genetic Insert: G6B
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713122
Comments: Article ref 10
Proper citation: RRID:Addgene_51644 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51775 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51774 Copy
Species: EBV and firefly
Genetic Insert: Luciferase-2A-EBNA-1
Vector Backbone Description: Backbone Marker:pHCAG generated from our laboratory; Backbone Size:7786; Vector Backbone:pHCAG amplicon vector; Vector Types:herpes simplex virus type-1 amplicon vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20963807
Comments: We would like to acknowledge, as a reference to depositing this plasmid, that the completed plasmid sequence is performed by Axil Scientific Sequencing , primer walking.
Addgene sequencing results find several discrepancies within the full plasmid sequence, they are not believed to effect the function of the plasmid.
Proper citation: RRID:Addgene_51783 Copy
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