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Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2171; Vector Backbone:ColE1 vector Omega1; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology, Assembly of GB2.0 transcriptional units; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: This is a BmsBI GB2.0 Omega entry vector. Any assembled unit(s) can be released with BsaI for further cloning into Alfa level vectors.
Proper citation: RRID:Addgene_118046 Copy
Species: Homo sapiens
Genetic Insert: 2 copies of the p53 DNA binding motif
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2343; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI
Proper citation: RRID:Addgene_118053 Copy
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2804; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: This is a GB2.0 domesticator vector. GBParts can be assembled in a BsmBI-ligation reaction. Any assembled Part can be released with BsaI for further cloning into Alpha level vectors.
Proper citation: RRID:Addgene_118043 Copy
Species: Synthetic
Genetic Insert: RBS, amilGFP
Vector Backbone Description: Backbone Marker:iGEM Registry; Vector Backbone:BioBrick pSB1C3 vector; Vector Types:Synthetic Biology, Escherichia coli; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29760772
Proper citation: RRID:Addgene_118006 Copy
Genetic Insert: ZB-linker-split C-terminal T7 RNAP expression plasmid
Vector Backbone Description: Vector Backbone:custom; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28192413
Proper citation: RRID:Addgene_118086 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pBC SK; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109061 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109058 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109059 Copy
Species: Synthetic
Genetic Insert: attB-Flag-DAS+4
Vector Backbone Description: Backbone Size:3139; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating Flag-DAS4+ fusions to mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109284 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Size:3082; Vector Backbone:pKM464; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating deletion of mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109301 Copy
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4008; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30798527
Proper citation: RRID:Addgene_109304 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109253 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109254 Copy
Species: Other
Genetic Insert: pARABAD-LUX operon
Vector Backbone Description: Vector Backbone:pSB1C3 (pMB1 origin of replication and CmR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109383 Copy
Vector Backbone Description: Vector Backbone: pLys (p15A origin of replication and CmR); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109386 Copy
Species: Other
Genetic Insert: TEV protease
Vector Backbone Description: Vector Backbone:pBROBE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30247489
Proper citation: RRID:Addgene_110206 Copy
Species: Homo sapiens
Genetic Insert: Elongin BC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.
Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.
Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...
2C9W: https://www.thesgc.org/structures/2C9W.
Proper citation: RRID:Addgene_110274 Copy
Genetic Insert: E. coli K-12 MG1655_rLP5(nth-ydgR)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29388765
Comments: Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
Proper citation: RRID:Addgene_110245 Copy
Genetic Insert: E. coli K-12 MG1655_fLP2(yieN-trkD)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29388765
Comments: Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
Proper citation: RRID:Addgene_110242 Copy
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