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  • Organism:drosophila melanogaster (facet)

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CJ20 (miR-1003 minigene)
 
Resource Report
Resource Website 		RRID:Addgene_17761 dme-miR-1003 minigene Drosophila melanogaster Ampicillin PMID:17589500 Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro. Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:09 0
CJ24 (let-7 minigene)
 
Resource Report
Resource Website 		RRID:Addgene_17764 dme-let-7 minigene Drosophila melanogaster Ampicillin PMID:17589500 let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin and cloned into pMT-puro. Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:09 0
CJ29 (let-7 perfect site)
 
Resource Report
Resource Website 		RRID:Addgene_17766 let-7 perfect site Drosophila melanogaster Ampicillin PMID:17589500 Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-ps (gagctcACTATACAACCTACTACCTCAactagt) Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells. Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:10 0
CJ32 (let-7 minigene)
 
Resource Report
Resource Website 		RRID:Addgene_17769 dme-let-7 minigene Drosophila melanogaster Ampicillin PMID:17589500 let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites. Backbone Marker:Carl Thummel Lab; Backbone Size:10200; Vector Backbone:pCaSpeR4-tubulin promoter; Vector Types:Drosophila P-element vector; Bacterial Resistance:Ampicillin 2026-08-15 01:11:10 0
CJ40 (let-7 seed mutant perfect site)
 
Resource Report
Resource Website 		RRID:Addgene_17773 let-7 seed mutant perfect site Drosophila melanogaster Ampicillin PMID:17589500 Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-psm (gagctcACTATACAACCTACAAGCACAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells. Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin seed mutant 2026-08-15 01:11:10 0
CJ49 (cg11094 utr)
 
Resource Report
Resource Website 		RRID:Addgene_17777 cg11094 utr fragment Drosophila melanogaster Ampicillin PMID:17589500 Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:12 0
CJ52 (cg1849 utr mut)
 
Resource Report
Resource Website 		RRID:Addgene_17780 cg1849 utr fragment mut Drosophila melanogaster Ampicillin PMID:17589500 Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin mutant version 2026-08-15 01:11:12 0
CJ53 (cg5166a/c utr)
 
Resource Report
Resource Website 		RRID:Addgene_17781 cg5166a/c utr fragment Drosophila melanogaster Ampicillin PMID:17589500 Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:11 0
CJ57 (cg6551 utr)
 
Resource Report
Resource Website 		RRID:Addgene_17783 cg6551 utr fragment Drosophila melanogaster Ampicillin PMID:17589500 Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:11 0
CJ58 (cg6551 utr mut)
 
Resource Report
Resource Website 		RRID:Addgene_17784 cg6551 utr fragment mut Drosophila melanogaster Ampicillin PMID:17589500 Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin mutant version 2026-08-15 01:11:12 0
pR70B04
 
Resource Report
Resource Website 		RRID:Addgene_165792 R70B04 Drosophila melanogaster Ampicillin PMID:34525356 Enhancer Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pDmVasa
 
Resource Report
Resource Website 		RRID:Addgene_165783 Vasa promoter Drosophila melanogaster Ampicillin PMID:34525356 Promoter Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pDmAlfaTub84b
 
Resource Report
Resource Website 		RRID:Addgene_165781 AlfaTub84b Drosophila melanogaster Ampicillin PMID:34525356 Promoter Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pDmHsp70b
 
Resource Report
Resource Website 		RRID:Addgene_165785 Hsp70b Drosophila melanogaster Ampicillin PMID:34525356 Promoter Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pDmHsp70b-TACT
 
Resource Report
Resource Website 		RRID:Addgene_165780 Hsp70b-TACT Drosophila melanogaster Ampicillin PMID:34525356 Promoter Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
p3xP3-Hsp70b
 
Resource Report
Resource Website 		RRID:Addgene_165773 3x3-Hsp70b Drosophila melanogaster Ampicillin PMID:34525356 Promoter Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pdSCP
 
Resource Report
Resource Website 		RRID:Addgene_165776 dSCP Drosophila melanogaster Ampicillin PMID:34525356 Mini-Prom Backbone Size:2790; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pDmActin5c
 
Resource Report
Resource Website 		RRID:Addgene_165777 Actin5c Drosophila melanogaster Ampicillin PMID:34525356 Promoter Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
p3xP3
 
Resource Report
Resource Website 		RRID:Addgene_165779 3xP3 Drosophila melanogaster Chloramphenicol PMID:34525356 Enhancer Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:09:31 0
hs-calx2
 
Resource Report
Resource Website 		RRID:Addgene_1741 calx2 Drosophila melanogaster Ampicillin PMID:15694324 Backbone Size:8780; Vector Backbone:pCaspeRhs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin N/A 2026-08-15 01:10:37 0

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