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Species: Homo sapiens
Genetic Insert: EYFP-p23
Vector Backbone Description: Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17010336
Proper citation: RRID:Addgene_108225 Copy
Species: Homo sapiens
Genetic Insert: EGFP-Hsp90b
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17010336
Proper citation: RRID:Addgene_108221 Copy
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang's Lab; Backbone Size:9175; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672770
Proper citation: RRID:Addgene_108294 Copy
Species: Other
Genetic Insert: Gal4 DBD-ERalpha HBD-VP16
Vector Backbone Description: Backbone Size:6408; Vector Backbone:pHCA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8370533
Proper citation: RRID:Addgene_108216 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108374 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108372 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108371 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108370 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108368 Copy
Species: Homo sapiens
Genetic Insert: Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108366 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108409 Copy
Species: Synthetic
Genetic Insert: xCas9 3.6
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108384 Copy
Species: Synthetic
Genetic Insert: xCas9(3.7)-BE4
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108381 Copy
Species: Synthetic
Genetic Insert: xCas9 3.7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108379 Copy
Species: Homo sapiens
Genetic Insert: PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108376 Copy
Species: Bos taurus
Genetic Insert: p110 KD
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6620; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10860837
Comments: A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR. The kinase dead mutant has an additional BamHI site at D915S.
Proper citation: RRID:Addgene_10837 Copy
Species: Bos taurus
Genetic Insert: p110
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6620; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10860837
Comments: A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR.
Proper citation: RRID:Addgene_10836 Copy
Species: Homo sapiens
Genetic Insert: Arf1 Q71L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11773070
Proper citation: RRID:Addgene_10832 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3457; Vector Backbone:pBluescript; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27913727
Comments: The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.
Proper citation: RRID:Addgene_108352 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_108342 Copy
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