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Species: Synthetic
Genetic Insert: LbCas12a Full Length Direct Repeat GC Swap crRNA
Vector Backbone Description: Backbone Size:3232; Vector Backbone:pBCSK+; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30892626
Comments: For cloning crRNA guide sequences into the GC swap frameworks: The top oligo should contain a GTGG overhang on its 5' end and the bottom oligo should contain AAAA overhang on its 5' end to match the overhangs that are produced by BbsI. In addition, for these constructs the guide oligos should encode the final two bases [GT] of the constant region of the crRNA prior to the spacer [targeting] sequence and then two additional Ts following the spacer sequence to ensure robust RNA Polymerase III termination.
Proper citation: RRID:Addgene_126643 Copy
Species: Synthetic
Genetic Insert: VHH[EGFP]
Vector Backbone Description: Vector Backbone:pCSDEST2; Vector Types:zebrafish muscle expression clone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_126749 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126904 Copy
Species: Synthetic
Genetic Insert: IV_CS10_1545_v1
Vector Backbone Description: Backbone Marker:Idem parts registry; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30990672
Comments: Due to the large repeat sequences, plasmid cannot be verified by NGS or Sanger sequencing. Addgene recommends digesting the plasmid for verification.
Proper citation: RRID:Addgene_126861 Copy
Species: Synthetic
Genetic Insert: IV_CS8_7569_v1
Vector Backbone Description: Backbone Marker:Idem parts registry; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30990672
Proper citation: RRID:Addgene_126865 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126902 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126900 Copy
Species: Synthetic
Genetic Insert: II_CS3_7560_v1
Vector Backbone Description: Backbone Marker:Eurofins; Vector Backbone:pBSSK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30990672
Proper citation: RRID:Addgene_126853 Copy
Species: Synthetic
Genetic Insert: IV_CS6_4323_v1
Vector Backbone Description: Backbone Marker:Idem parts registry; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30990672
Proper citation: RRID:Addgene_126859 Copy
Species: Synthetic
Genetic Insert: SomArchon
Vector Backbone Description: Backbone Size:4813; Vector Backbone:pAAV-CaMKII; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597963
Proper citation: RRID:Addgene_126942 Copy
Species: Synthetic
Genetic Insert: SomArchon
Vector Backbone Description: Backbone Size:5052; Vector Backbone:pAAV-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597963
Proper citation: RRID:Addgene_126943 Copy
Species: Synthetic
Genetic Insert: SomArchon
Vector Backbone Description: Backbone Size:5279; Vector Backbone:pAAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597963
Proper citation: RRID:Addgene_126941 Copy
Species: Synthetic
Genetic Insert: SomArchon-P2A-CoChR-Kv2.1
Vector Backbone Description: Backbone Size:5279; Vector Backbone:pAAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597963
Proper citation: RRID:Addgene_126945 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.
Proper citation: RRID:Addgene_126890 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126895 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126897 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.
Proper citation: RRID:Addgene_126883 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126881 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126882 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.
Proper citation: RRID:Addgene_126887 Copy
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