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Species: Lobophyllia hemprichii
Genetic Insert: mEos3.2
Vector Backbone Description: Vector Backbone:pMT85-2res; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35638916
Proper citation: RRID:Addgene_173894 Copy
Species: Lobophyllia hemprichii
Genetic Insert: mEos3.2
Vector Backbone Description: Vector Backbone:pMT85-2res; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35638916
Proper citation: RRID:Addgene_173895 Copy
Vector Backbone Description: Backbone Size:3052; Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34608327
Proper citation: RRID:Addgene_174039 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Zebrafish knock-in tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34495314
Comments: Not all restriction sites in the multiple cloning sites are single cutters, depending on the fluorescent protein sequence of the insert within the plasmid.
Proper citation: RRID:Addgene_174024 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2294; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17398 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9150; Vector Backbone:pQCXIN; Vector Types:Retroviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17399 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21267423
Proper citation: RRID:Addgene_174006 Copy
Species: Homo sapiens
Genetic Insert: Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173952 Copy
Species: Homo sapiens
Genetic Insert: Enhancer regions 1 and 2 (S1 and S2) from SOX9 enhancer cluster EC1.35
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173953 Copy
Species: Homo sapiens
Genetic Insert: CCND1
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:VectorBuilder pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174155 Copy
Species: Homo sapiens
Genetic Insert: AMBRA1
Vector Backbone Description: Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174157 Copy
Species: Homo sapiens
Genetic Insert: Control sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174149 Copy
Species: Synthetic
Genetic Insert: dCas9, 10xSunTag, tagBFP
Vector Backbone Description: Backbone Size:5706; Vector Backbone:AmpR-Ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31941101
Comments: Shorter version of the original plasmid, in which a 4.5-kb fragment in the vector backbone was deleted
Proper citation: RRID:Addgene_174141 Copy
Species: Homo sapiens
Genetic Insert: Control sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174148 Copy
Vector Backbone Description: Backbone Size:13430; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32976518
Comments: Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098
Please note there are two mutations in HygR- E180D and A95S. Depositor confirms these do not affect plasmid function.
Proper citation: RRID:Addgene_174050 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Size:7900; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270926
Proper citation: RRID:Addgene_174168 Copy
Species: synthetic
Genetic Insert: Cas9-BFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34697476
Comments: Please visit https://www.biorxiv.org/content/10.1101/775080v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_174162 Copy
Species: Synthetic
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:5653; Vector Backbone:pRBC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31243963
Proper citation: RRID:Addgene_174076 Copy
Species: Mus musculus
Genetic Insert: Let-7g mutant
Vector Backbone Description: Backbone Marker:Jacks Lab; Backbone Size:9200; Vector Backbone:Puro.Cre; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18308936
Comments: Puro.let7gsm.Cre was generated by two rounds site-directed mutagenesis of Puro.let7g.Cre with mut1F (5’-GATTCCAGGCTGACCTAGTAGTTTGTACAGTTTGAGGGTCTATGATAC-3’), mut1R (5’-GTATCATAGACCCTCAAACTGTACAAACTACTAGGTCAGCCTGGAATC-3’), mut2F (5’-CAGGAGATAACTGTACAGGCCACTGGGTTGCCAGGAAC-3’), and mut2R (5’-GTTCCTGGCAACCCAGTGGCCTGTACAGTTATCTCCTG-3’).
For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
Proper citation: RRID:Addgene_17410 Copy
Species: Mus musculus
Genetic Insert: HMGA2
Vector Backbone Description: Backbone Marker:Available at Addgene (#1766); Backbone Size:4900; Vector Backbone:pBabe-Zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18308936
Comments: The ORF of human HMGA2 was amplified using HMGA2F (5’-TAAGGATCCATGAGCGCACGCGGTGAG-3’) and HMGA2R (5’- GCGGAATTCCTAATCCTCCTCTGCGGACTCTTG-3’). The product was cut with BamHI and EcoRI and cloned into pBabe.Zeo.
Proper citation: RRID:Addgene_17411 Copy
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