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Genetic Insert: 3 copies of the CXCR4 small RNA target site
Vector Backbone Description: Backbone Marker:Sharp Lab (http://web.mit.edu/sharplab/RNAi/Cloning.html); Vector Backbone:mutpRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21878547
Proper citation: RRID:Addgene_40758 Copy
Species: Mus musculus
Genetic Insert: Mouse vasopressin gene
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSE280; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12944509
Comments: the following mutations are known and do not effect plasmid function: TT--5510CTTG, G5386A, A4984T,A6794G.
Proper citation: RRID:Addgene_40865 Copy
Species: Drosophila melanogaster
Genetic Insert: Four target sites perfectly complementary to let-7
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20558712
Comments: The let-7 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTA GAA CTA TAC AAC CTA CTA CCT CAT CTA GAA CTA TAC AAC CTA CTA CCT CAT
as: CTA GAT GAG GTA GTA GGT TGT ATA GTT CTA GAT GAG GTA GTA GGT TGT ATA GTT
This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
Proper citation: RRID:Addgene_40834 Copy
Species: Mus musculus
Genetic Insert: Dppa2
Vector Backbone Description: Backbone Size:8339; Vector Backbone:FUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980981
Proper citation: RRID:Addgene_40799 Copy
Species: Drosophila melanogaster
Genetic Insert: Two target sites perfectly complementary to miR-1
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20558712
Comments: The miR-1 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTA GAC TCC ATA CTT CTT TAC ATT CCA TCT AGA CTC CAT ACT TCT TTA CAT TCC AT
as: CTA GAT GGA ATG TAA AGA AGT ATG GAG TCT AGA TGG AAT GTA AAG AAG TAT GGA GT
This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
Proper citation: RRID:Addgene_40832 Copy
Species: Mus musculus
Genetic Insert: Esrrb
Vector Backbone Description: Backbone Size:8339; Vector Backbone:FUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980981
Proper citation: RRID:Addgene_40798 Copy
Species: Drosophila melanogaster
Genetic Insert: three sites partially complementary to miR-34
Vector Backbone Description: Backbone Marker:promega; Backbone Size:6273; Vector Backbone:psiCheck-2; Vector Types:Insect Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22055293
Comments: The psiCheck-3xmiR-34 sequences were cloned by synthesized dsDNA
S: 5'-TCGAGGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGC
AS: 5'-GGCCGCTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACC
the triple(3x)-repeated sequence:
5'TGGCAGTGACCTTAGCATCAACAC-3'
3'ACCGTCACTGGAATCGTAGTTGTG-5'
pairs with dme-miR-34 (uggcagugugguuagcugguugug) with "seed(red) plus 3' complementary region (blue)" fashion.
Proper citation: RRID:Addgene_40831 Copy
Species: Homo sapiens
Genetic Insert: SNCA
Vector Backbone Description: Backbone Marker:Roche; Backbone Size:5450; Vector Backbone:pHM6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698681
Proper citation: RRID:Addgene_40825 Copy
Species: Homo sapiens
Genetic Insert: SNCA
Vector Backbone Description: Backbone Marker:Roche; Backbone Size:5450; Vector Backbone:pHM6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698681
Proper citation: RRID:Addgene_40824 Copy
Species: Xanthamonas oryzae
Genetic Insert: Transcription Activator Like Effector Nuclease
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Comments: MR15 is a designation vector for the Cermak et al Golden Gate system designed for expression in mammalian cells.
Please acknowledge the principal investigator, Dr. Gang Bao, and include this article in your citations if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 40788" in your Materials and Methods section.
Proper citation: RRID:Addgene_40788 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14701799
Comments: Site-directed mutagenesis was used to to introduce a stop codon in pEBB XIAP at AA 449 (AAG to TGA).
(#721)
Proper citation: RRID:Addgene_40828 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17114285
Proper citation: RRID:Addgene_40813 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17114285
Proper citation: RRID:Addgene_40812 Copy
Species: Homo sapiens
Genetic Insert: MAP2K1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8052857
Proper citation: RRID:Addgene_40811 Copy
Species: Homo sapiens
Genetic Insert: Braf
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pcDNA3.1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27870838
Proper citation: RRID:Addgene_40775 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11591711
Proper citation: RRID:Addgene_40819 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11591711
Proper citation: RRID:Addgene_40818 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11591711
Proper citation: RRID:Addgene_40817 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17114285
Proper citation: RRID:Addgene_40816 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17114285
Proper citation: RRID:Addgene_40815 Copy
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