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Species: Zea mays (maize)
Genetic Insert: MLKG1
Vector Backbone Description: Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30659121
Proper citation: RRID:Addgene_130834 Copy
Species: Zea mays (maize)
Genetic Insert: SUN2
Vector Backbone Description: Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30659121
Proper citation: RRID:Addgene_130831 Copy
Species: Zea mays (maize)
Genetic Insert: MLKP2
Vector Backbone Description: Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30659121
Proper citation: RRID:Addgene_130832 Copy
Species: Zea mays (maize)
Genetic Insert: MLKS2
Vector Backbone Description: Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31221013
Proper citation: RRID:Addgene_130864 Copy
Species: Zea mays (maize)
Genetic Insert: MLKS2
Vector Backbone Description: Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31221013
Proper citation: RRID:Addgene_130862 Copy
Species: E.coli
Genetic Insert: tetR gene by pFRA61 (Boldrin et al 2010)
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pSM128 derived plasmid (the region carrying the attB site and the integrase gene was previously removed using NheI and HindIII); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30962489
Comments: The plasmid also carries attP sequence belonging to pMC1s plasmid and PfurA 102 tetO- pip belonging to pFRA61 (Boldrin et al 2010).
Proper citation: RRID:Addgene_131106 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-3
Proper citation: RRID:Addgene_136372 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-4
Proper citation: RRID:Addgene_136373 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: sgRNA scaffold and MS2 sequence are from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-5
Proper citation: RRID:Addgene_136374 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Proper citation: RRID:Addgene_136377 Copy
Species: Francisella novicida U112
Genetic Insert: dFnCas12a-TV
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-0
Proper citation: RRID:Addgene_136379 Copy
Species: Alicyclobacillus acidiphilus
Genetic Insert: AaCas12b (D570A)-TV-T2A-MCP-TV
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-2
Proper citation: RRID:Addgene_136381 Copy
Species: Alicyclobacillus acidiphilus
Genetic Insert: AaCas12b (D570A)-TV-T2A-MCP-VPR
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170285
Comments: TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-3; VPR activation system is from: Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, E PRI, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12(4):326–8. 10.1038/nmeth.3312
Proper citation: RRID:Addgene_136382 Copy
Vector Backbone Description: Backbone Size:7912; Vector Backbone:N/A; Vector Types:E. coli-S. arueus shuttle vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:9045821
Proper citation: RRID:Addgene_13656 Copy
Species: M13 bacteriophage
Genetic Insert: gIII
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32042170
Comments: TAA PAM
Proper citation: RRID:Addgene_136928 Copy
Genetic Insert: mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137075 Copy
Genetic Insert: mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137074 Copy
Genetic Insert: mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137077 Copy
Genetic Insert: mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137076 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin-like-conjugating enzyme ATG3
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31315929
Proper citation: RRID:Addgene_129293 Copy
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