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Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110086 Copy
Species: Danio rerio
Genetic Insert: His6-HSPB7
Vector Backbone Description: Backbone Size:5186; Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29397684
Comments: Thrombin cleavage site between N-terminal His tag and protein
Proper citation: RRID:Addgene_110071 Copy
Species: Danio rerio
Genetic Insert: His6-HSPB7
Vector Backbone Description: Backbone Size:5186; Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29397684
Comments: Thrombin cleavage site between N-terminal His tag and protein
Proper citation: RRID:Addgene_110072 Copy
Species: Synthetic
Genetic Insert: L4
Vector Backbone Description: Vector Backbone:pET30; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27548700
Proper citation: RRID:Addgene_110068 Copy
Species: Synthetic
Genetic Insert: L2
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27548700
Proper citation: RRID:Addgene_110067 Copy
Species: Other
Genetic Insert: VPR, TEVp
Vector Backbone Description: Vector Backbone:HIV-1_VPR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26300212
Proper citation: RRID:Addgene_110200 Copy
Species: Sulfolobus acidocaldarius
Genetic Insert: GGR
Vector Backbone Description: Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24954619
Proper citation: RRID:Addgene_110152 Copy
Species: Sulfolobus acidocaldarius
Genetic Insert: GGR
Vector Backbone Description: Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24954619
Proper citation: RRID:Addgene_110151 Copy
Species: Klebsiella oxytoca ATCC 8724
Genetic Insert: pduCDE
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5238; Vector Backbone:pET-29 b (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29526088
Proper citation: RRID:Addgene_110138 Copy
Species: Homo sapiens
Genetic Insert: hCCR5
Vector Backbone Description: Vector Backbone:mTFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30150645
Proper citation: RRID:Addgene_110193 Copy
Species: Homo sapiens
Genetic Insert: hCXCR4
Vector Backbone Description: Vector Backbone:mTFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37591879
Proper citation: RRID:Addgene_110196 Copy
Species: Homo sapiens
Genetic Insert: hCCR5
Vector Backbone Description: Vector Backbone:LSSmOrange-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37591879
Proper citation: RRID:Addgene_110194 Copy
Species: Rattus norvegicus
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20628354
Proper citation: RRID:Addgene_110182 Copy
Species: Roseburia inulinivorans strain A2-194
Genetic Insert: Propanediol Dehydratase Activating Enzyme
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5292; Vector Backbone:pET-28 a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28183913
Proper citation: RRID:Addgene_110180 Copy
Species: Mus musculus
Genetic Insert: MeCP2_e2 FL [R111G] (mouse cDNA)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4680; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29019980
Proper citation: RRID:Addgene_110187 Copy
Species: Clostridium difficile 70-100-2010
Genetic Insert: Pyrroline-5-carboxylate Reductase
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5292; Vector Backbone:pET-28 a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28183913
Proper citation: RRID:Addgene_110295 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Opher Gileadi, Addgene plasmid #26107; Vector Backbone:pNIC-ZB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27871366
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110219 Copy
Species: Synthetic
Genetic Insert: gHH_44
Vector Backbone Description: Backbone Size:5919; Vector Backbone:pCDB364; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30152054
Proper citation: RRID:Addgene_110276 Copy
Species: Homo sapiens
Genetic Insert: WNK2
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21(DE3)-R3-pRARE2 for expression. 6ELM: https://www.thesgc.org/structures/6ELM.
Proper citation: RRID:Addgene_110250 Copy
Species: Homo sapiens
Genetic Insert: KALRN
Vector Backbone Description: Vector Backbone:pNIC28-10His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21 (DE3) Star for expression. PDB structure is a complex of (KALRNA-c001 & RAC1A-c007). 5o33: https://www.thesgc.org/structures/5o33.
Proper citation: RRID:Addgene_110256 Copy
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