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Species: Mus musculus
Genetic Insert: Calcium channel, voltage-dependent, L type, alpha 1C subunit
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267232
Comments: GenBank Accesssion number: AY728090
Proper citation: RRID:Addgene_26572 Copy
Species: Mus musculus
Genetic Insert: Par6a
Vector Backbone Description: Backbone Size:6525; Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18922891
Proper citation: RRID:Addgene_26668 Copy
Species: Mus musculus
Genetic Insert: Flag Par6a
Vector Backbone Description: Backbone Size:6525; Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18922891
Proper citation: RRID:Addgene_26666 Copy
Species: Mus musculus
Genetic Insert: Δ90beta catenin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Δ90AEGFP is Δ90betacatenin fused with EGFP-mut4 from Ami Okada
Proper citation: RRID:Addgene_26644 Copy
Species: Mus musculus
Genetic Insert: Cortactin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16280362
Proper citation: RRID:Addgene_26723 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, reporter for Rho; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15684032
Comments: The RhoA-binding domain of rhotekin (rGBD) was fused to eGFP in order to visualize active RhoA
Proper citation: RRID:Addgene_26732 Copy
Species: Mus musculus
Genetic Insert: Actin-binding Protein-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19910490
Proper citation: RRID:Addgene_26719 Copy
Species: Mus musculus
Genetic Insert: MyoD-ER(T)
Vector Backbone Description: Backbone Size:6707; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511457
Comments: Cloning of the MyoD-ER(T):
Briefly, the full-length mouse MyoD cDNA was isolated and cloned from a previously described adenoviral vector (Lattanzi et al., 1998). A cDNA for the modified estrogen receptor responsive to tamoxifen and 4-hydroxytamoxifen, ER(T) (Danielian et al., 1993), was cloned using a PCR method with the primer pair including an internal ClaI restriction enzyme site: (sense) 5′-ACGGCATCGATTCTGCTGGAGACATGAGAGCT-3′, and (anti-sense) 5′-GCGCCATCGATGACTGTGGCAGGGAAACCCT-3′, and then inserted into the NarI site (amino acid 173) in the middle of the mouse MyoD cDNA. The final clone was verified by DNA sequencing.
There is a mutation G525R in the mouse estrogen receptor; the mutation confers selective 4-hydroxytamoxifen sensitivity (vs. estradiol) (Danielian et al. 1993 Molec Endocrin 7: 232-240).
Proper citation: RRID:Addgene_26809 Copy
Species: Mus musculus
Genetic Insert: MyoD
Vector Backbone Description: Backbone Marker:Lentiviral plasmid backbone from L. Naldini; Backbone Size:6707; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511457
Comments: CMV promotor
Proper citation: RRID:Addgene_26808 Copy
Species: Mus musculus
Genetic Insert: autophagy-related 3 (yeast)
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20723759
Proper citation: RRID:Addgene_26926 Copy
Species: Mus musculus
Genetic Insert: NICD
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508304
Proper citation: RRID:Addgene_26891 Copy
Species: Mus musculus
Genetic Insert: Hes5 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:0; Vector Backbone:PGV-B; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Comments: Contains the Hes5 promoter (-685 to +28).
C->T mutation in the center of the promoter does affect function.
Proper citation: RRID:Addgene_26869 Copy
Species: Mus musculus
Genetic Insert: Hes5 promoter
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4200; Vector Backbone:pDsRed2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Comments: Hes5p from -685 to +28.
Proper citation: RRID:Addgene_26868 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: Kozak modified mouse M33 cDNA, N-terminal flag. (kingston #1390)
Proper citation: RRID:Addgene_1992 Copy
Species: Mus musculus
Genetic Insert: Ring1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: Kozak modified mouse RING1A cDNA, N-terminal flag. (kingston #1393)
Proper citation: RRID:Addgene_1995 Copy
Species: Mus musculus
Genetic Insert: 2xGfaABC1D-tTA
Vector Backbone Description: Backbone Size:7425; Vector Backbone:pTYF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18324737
Proper citation: RRID:Addgene_19981 Copy
Species: Mus musculus
Genetic Insert: AID 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2000; Vector Backbone:pGEM-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18450484
Proper citation: RRID:Addgene_19986 Copy
Species: Mus musculus
Genetic Insert: Nfkb2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20019 Copy
Species: Mus musculus
Genetic Insert: RelB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20017 Copy
Species: Mus musculus
Genetic Insert: RelA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16166378
Comments: See cFlag pcDNA plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20012 Copy
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