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Species: Homo sapiens
Genetic Insert: YAP1-Sh1
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain (Addgene plasmid # 21915); Backbone Size:8768; Vector Backbone:Tet-pLKO-Puro (pLKO-Tet-On); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Proper citation: RRID:Addgene_176846 Copy
Species: Homo sapiens
Genetic Insert: hPLXNB2
Vector Backbone Description: Backbone Marker:Eric Campeau (Addgene plasmid # 26432); Backbone Size:9185; Vector Backbone:pLenti CMVtight Neo DEST (w770-1); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Proper citation: RRID:Addgene_176849 Copy
Species: Homo sapiens
Genetic Insert: hPLXNB2
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 19067); Backbone Size:9778; Vector Backbone:pLENTI PGK Neo DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Comments: This is the wild-type full length cDNA sequence for PLXNB2. This plasmid is similar to Addgene plasmid # 86237, but is not CRISPR-resistant.
Proper citation: RRID:Addgene_176848 Copy
Species: Homo sapiens
Genetic Insert: Human CYP3A4 proximal enhancer sgRNA for activation
Vector Backbone Description: Vector Backbone:lenti-sgRNA(MS2)_zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35452598
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.24.485641v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176839 Copy
Species: Homo sapiens
Genetic Insert: DOP1B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: DOP1B (BC156374.1) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176979 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698938
Comments: PTPalpha-HA was amplified from plasmid pXJ41PTPalpha using the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGCACGTCGTATGGGTACTTGAAGT- TGGCATAATCTGA-3'. The 5' primer contains a HindIII site, a Kozak consensus sequence CCACC and the start codon. The 3' primer contains the coding sequence for the HA epitope YPYDVPDYA, the stop codon and a HindIII site. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk (Life Technologies), it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17691 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha CCSS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698938
Comments: The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17692 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha Y789F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698938
Comments: PTPalpha(Y789F)-HA was generated using pXJ41PTPalpha as a template with the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGGCACGTCGTATGGGTACTTGAAGTTGGCAAAATCTGA-3'. The substitution ATA->AAA results in a Tyr789->Phe substitution. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17693 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha S204A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923305
Comments: See article for cloning details. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17694 Copy
Species: Homo sapiens
Genetic Insert: MOSMO
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: MOSMO (NM_001164579.2) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176975 Copy
Species: Homo sapiens
Genetic Insert: RUNX1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: RUNX1 (BC136381) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176974 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha S204A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923305
Comments: See article for cloning details. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17699 Copy
Species: Homo sapiens
Genetic Insert: GLI1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: GLI1 (NM_005269.2) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176968 Copy
Species: Homo sapiens
Genetic Insert: CHAF1B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: CHAF1B (NM_005441) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176969 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Size:5022; Vector Backbone:pLIB-HIS-TEV; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791727
Proper citation: RRID:Addgene_177012 Copy
Species: Homo sapiens
Genetic Insert: CBR3
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: CBR3 (NM_001236) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176961 Copy
Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: SOD1 (NM_000454) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176964 Copy
Species: Homo sapiens
Genetic Insert: CLDN8
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: CLDN8 (NM_199328) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176963 Copy
Species: Homo sapiens
Genetic Insert: GABPA
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Proper citation: RRID:Addgene_176999 Copy
Species: Homo sapiens
Genetic Insert: GNAS
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Proper citation: RRID:Addgene_176991 Copy
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