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  • Bacterial Resistance:spectinomycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
GFP-PK1Dexp
 
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RRID:Addgene_130834 MLKG1 Zea mays (maize) Spectinomycin PMID:30659121 Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:12 0
pHWBF06exp
 
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RRID:Addgene_130831 SUN2 Zea mays (maize) Spectinomycin PMID:30659121 Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:12 0
pHWBF07exp
 
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RRID:Addgene_130832 MLKP2 Zea mays (maize) Spectinomycin PMID:30659121 Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:12 0
MLKS2delARMexp
 
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RRID:Addgene_130864 MLKS2 Zea mays (maize) Spectinomycin PMID:31221013 Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin Deletion of armadillo domain 2026-08-15 01:05:12 0
MLKS2delKASHexp
 
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RRID:Addgene_130862 MLKS2 Zea mays (maize) Spectinomycin PMID:31221013 Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin Deletion of KASH domain 2026-08-15 01:05:12 0
pANTO5
 
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RRID:Addgene_131106 tetR gene by pFRA61 (Boldrin et al 2010) E.coli Spectinomycin PMID:30962489 The plasmid also carries attP sequence belonging to pMC1s plasmid and PfurA 102 tetO- pip belonging to pFRA61 (Boldrin et al 2010). Backbone Size:7500; Vector Backbone:pSM128 derived plasmid (the region carrying the attB site and the integrase gene was previously removed using NheI and HindIII); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:05:14 0
pYPQ141-ZmUbi-RZ-Aa1.2.3
 
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RRID:Addgene_136372 Spectinomycin PMID:32170285 sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-3 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:06 0
pYPQ141-ZmUbi-RZ-Aa1.2
 
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RRID:Addgene_136373 Spectinomycin PMID:32170285 sgRNA scaffold is from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-4 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:05 0
pYPQ141-ZmUbi-RZ-Aa3.8.3
 
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RRID:Addgene_136374 Spectinomycin PMID:32170285 sgRNA scaffold and MS2 sequence are from: Teng, F., Cui, T., Feng, G. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov 4, 63 (2018) doi:10.1038/s41421-018-0069-5 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:05 0
pYPQ141-ZmUbi-RZ-Aac.3
 
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RRID:Addgene_136377 Spectinomycin PMID:32170285 Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:05 0
pYPQ239A (dFnCas12a)-TV
 
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RRID:Addgene_136379 dFnCas12a-TV Francisella novicida U112 Spectinomycin PMID:32170285 TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-0 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D917A 2026-08-15 01:06:05 0
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
 
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RRID:Addgene_136381 AaCas12b (D570A)-TV-T2A-MCP-TV Alicyclobacillus acidiphilus Spectinomycin PMID:32170285 TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-2 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D570A 2026-08-15 01:06:05 0
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
 
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RRID:Addgene_136382 AaCas12b (D570A)-TV-T2A-MCP-VPR Alicyclobacillus acidiphilus Spectinomycin PMID:32170285 TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-3; VPR activation system is from: Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, E PRI, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12(4):326–8. 10.1038/nmeth.3312 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin D570A 2026-08-15 01:06:05 0
pCL52.2
 
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RRID:Addgene_13656 Spectinomycin PMID:9045821 Backbone Size:7912; Vector Backbone:N/A; Vector Types:E. coli-S. arueus shuttle vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:07 0
pTW199b2
 
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RRID:Addgene_136928 gIII M13 bacteriophage Spectinomycin PMID:32042170 TAA PAM Vector Backbone:ColE1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin none 2026-08-15 01:06:11 0
pL0M-S-mNeonGreen-EC18153
 
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1+ mentions
RRID:Addgene_137075 mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning Spectinomycin PMID:32072132 If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected]. Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint. Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:13 6
pL0M-S-mTurquoise2-EC18152
 
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RRID:Addgene_137074 mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning Spectinomycin PMID:32072132 If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected]. Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint. Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:13 0
pL0M-S-mKOκ-EC18154
 
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RRID:Addgene_137077 mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning Spectinomycin PMID:32072132 If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected]. Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint. Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:13 0
pL0M-S-mClover3-EC18157
 
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RRID:Addgene_137076 mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning Spectinomycin PMID:32072132 If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected]. Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint. Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:06:13 0
pDONR223 ATG3 C264A
 
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RRID:Addgene_129293 Ubiquitin-like-conjugating enzyme ATG3 Homo sapiens Spectinomycin PMID:31315929 Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin changed Cysteine 264 to Alanine 2026-08-15 01:04:56 0

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