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Species: Synthetic
Genetic Insert: UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC19; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Comments: Cloning was via SLiCE (Seamless Ligation Cloning Extract).
Note: The right tol1 region contains a single base pair mismatch compared to the depositor's expected sequence. The depositor has noted that this could reduce transgenesis efficiency, although they were able to successfully establish lines using the parent vector.
Proper citation: RRID:Addgene_108356 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT integrating plasmid for creating knockouts of target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108322 Copy
Species: Synthetic
Genetic Insert: RspCas13d
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5390; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29551514
Comments: For commercial use, please contact Arbor Biotechnologies at [email protected].
Proper citation: RRID:Addgene_108305 Copy
Species: A. victoria
Genetic Insert: GFP under control of arabinose-inducible promoter pBAD
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:3541; Vector Backbone:pJ401; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.20.449138v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108315 Copy
Species: mycobacterial phage
Genetic Insert: Che9C RecT
Vector Backbone Description: Backbone Marker:Dirk Schnappinger and Sabine Ehrt; Vector Backbone:pUV15tet-O; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30538179
Comments: The plasmid is induced with 500 ng/ml anhydrotetracycline to induce expression of RecT and Bxb1 Integrase for performing the ORTBIT protocol.
Proper citation: RRID:Addgene_108319 Copy
Species: Homo sapiens
Genetic Insert: Shugoshin 1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25892238
Proper citation: RRID:Addgene_108494 Copy
Species: Homo sapiens
Genetic Insert: AuroraB
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22267324
Comments: Addgene's sequencing results found M298T in AuroraB compared to the NCBI reference. The depositing lab states that there is a SNP at this location, and the discrepancy should not affect plasmid function.
Proper citation: RRID:Addgene_108493 Copy
Species: Homo sapiens
Genetic Insert: AuroraB
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22267324
Comments: Addgene's sequencing results found M298T in AuroraB compared to the NCBI reference. The depositing lab states that there is a SNP at this location, and the discrepancy should not affect plasmid function.
Proper citation: RRID:Addgene_108488 Copy
Species: Homo sapiens
Genetic Insert: INCENP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19150808
Proper citation: RRID:Addgene_108487 Copy
Species: Homo sapiens
Genetic Insert: SOST
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15908424
Proper citation: RRID:Addgene_10842 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pCAG-WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Comments: Addgene QC NGS identified some sequence discrepancies in the plasmid backbone, relative to the depositing lab's provided sequence. Please see the Sequences webpage for additional information.
Proper citation: RRID:Addgene_108423 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pAAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108422 Copy
Species: Human papillomavirus
Genetic Insert: HPV11 E6
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1661671
Comments: See author's map.
Proper citation: RRID:Addgene_10848 Copy
Species: Mus musculus
Genetic Insert: NMHC II-C
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14594953
Proper citation: RRID:Addgene_10843 Copy
Species: Synthetic
Genetic Insert: zArchon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pTol2-UAS; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108427 Copy
Species: Synthetic
Genetic Insert: attB-eGFP-4xGly-TEV-Flag-6xHis
Vector Backbone Description: Backbone Marker:Kenan Murphy; Backbone Size:3159; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating EGFP fusions to target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108434 Copy
Species: Synthetic
Genetic Insert: TetR + PTet*-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108529 Copy
Species: Synthetic
Genetic Insert: Telomere-targeting sgRNA
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29735996
Comments: CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529–537.
Proper citation: RRID:Addgene_108649 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hyBRET-ERK-EV
Vector Backbone Description: Backbone Size:4739; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29895862
Proper citation: RRID:Addgene_108652 Copy
Species: Homo sapiens
Genetic Insert: HHR23A
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX-4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10373495
Comments: See author's map.
Proper citation: RRID:Addgene_10864 Copy
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