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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Linked Free PcPTS
 
Resource Report
Resource Website
RRID:Addgene_182486 FPPS-PTS Saccharomyces cerevisiae Ampicillin PMID:36990382 Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:19 0
Coexpressed Free NES
 
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RRID:Addgene_182482 FPPS Saccharomyces cerevisiae Ampicillin PMID:36990382 Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:19 0
Linked Free ClLIS1 (M)
 
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RRID:Addgene_182487 FPPS(M)-LS Saccharomyces cerevisiae Ampicillin PMID:36990382 Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:19 0
Coexpressed Free GFP-NES
 
Resource Report
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RRID:Addgene_182491 FPPS Saccharomyces cerevisiae Ampicillin PMID:36990382 Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:19 0
Linked Free (PT)4P
 
Resource Report
Resource Website
RRID:Addgene_182490 FPPS-NES Saccharomyces cerevisiae Ampicillin PMID:36990382 Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:19 0
Coexpressed Free deadFPPS-NES
 
Resource Report
Resource Website
RRID:Addgene_182492 FPPS Saccharomyces cerevisiae Ampicillin PMID:36990382 deadFPPS sequence also contains G253D mutation that was inadvertently introduced during cloning. Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:19 0
pFA6a-HISMX6-pIME2
 
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RRID:Addgene_191793 HIS3 pIME2 Saccharomyces cerevisiae Ampicillin Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-kanMX6-pSPR3
 
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RRID:Addgene_191788 KanMX pSPR3 Saccharomyces cerevisiae Ampicillin Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-kanMX6-pSPO11
 
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RRID:Addgene_191767 KanMX pSPO11 Saccharomyces cerevisiae Ampicillin Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
Coexpressed VLP NES (G)
 
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RRID:Addgene_182477 VP2C-FPPS(G) Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Coexpressed Free NES (M)
 
Resource Report
Resource Website
RRID:Addgene_182437 FPPS(M) Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Linked Free NES (M)
 
Resource Report
Resource Website
RRID:Addgene_182436 FPPS(M)-NES Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
pESC-NAT-Ago-Dcr
 
Resource Report
Resource Website
RRID:Addgene_160381 Ago- Dcr Saccharomyces cerevisiae Ampicillin AGO1 and DCR1 genes were amplified from S. castellii gDNA using primersatac gcggccgc aacgatgtcatccaattcgg+ and atac gcggccgc agcacataaactctttgttc, respectively. Plasmid pESC-LEU was converted to leu2::NAT derivative via short-homology in vivo replacement using PCR product generated using primers AAGCAAGGATTTTCTTAACTTCTTCGGCGACAGCATCACCGACTTCGGTGc agc tga agc ttc gta cgc+tttttccaataggtggttagcaatcgtcttactttctaacttttcttacct agg cca cta gtg gat ctg and pAG25 template. AGO1 was cloned into NotI site of pESC-NAT to yield pESC-NAT-AGO1 plasmid. DCR1 was cloned into ApaI/XhoI site of pESC-NAT-AGO1 to yield pESC-NAT-AGO1-DCR1 plasmid. Backbone Size:7959; Vector Backbone:pESC; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:24:29 0
N1199 launching
 
Resource Report
Resource Website
RRID:Addgene_160386 N1199 Saccharomyces cerevisiae Ampicillin The plasmid was constructed by gene synthesis and cloning by Genewiz. Backbone Size:4984; Vector Backbone:PSV3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:24:29 0
pGAL-PDE1
 
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RRID:Addgene_160388 PDE1 Saccharomyces cerevisiae Ampicillin Please note plasmid contains the following mutations T114A, K153E, I201T and N306S in PDE1. Depositor confirms plasmid is functional. Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:3825; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:24:29 0
PJD1223
 
Resource Report
Resource Website
RRID:Addgene_160390 L-A Gag-Pol Saccharomyces cerevisiae Ampicillin pJD1223-NAT was constructed by short-homology in vivo replacement of TRP1 on pJD1223 (J Virol . 1993 May;67(5):2764-71) with NAT PCR product generated using primers TATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTcagctgaagcttcgtacgc and caagtgcacaaacaatacttaaataaatactactcagtaataacctattttaggccactagtggatctg from pAG25. Backbone Marker:J Virol . 1993 May;67(5):2764-71. doi: 10.1128/JVI.67.5.2764-2771.1993.; Backbone Size:9854; Vector Backbone:PJD1223; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:24:30 0
pAG25-1199 cDNA
 
Resource Report
Resource Website
RRID:Addgene_160379 N1199 Saccharomyces cerevisiae Ampicillin 2.8 kb dsRNA species gel purified from yJM 1199 and ligated to PC3 oligo (/5Phos/GG ATC CCG GGA ATT CGG TAA TAC GAC TCA CTA TAT TTT TAT AGT GAG TCG TAT TA). After reverse transcription and strand annealing, cDNA amplified with PC2 primer (CCG AAT TCC CGG GAT CC )to yield a 2.8 kb fragment. Fragment phosphorylated and ligated to pAG25 digested with EcoRV and phosphatase treated. Clones checked with restriction digest and sequencing. Near full length N1199 cDNA Backbone Size:3704; Vector Backbone:pAG25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:24:30 0
p404-Grx5-GFP1-10
 
Resource Report
Resource Website
RRID:Addgene_91952 Grx5-GFP1-10 Saccharomyces cerevisiae Ampicillin PMID:28241148 Split GFP was synthesized using gBlocks based on these references: Stephanie Cabantous et al., 2005 Nature Methods and Christine J. Smoyer et al. 2016 JCB. Backbone Marker:PMID: 7737504; Vector Backbone:p404TDH3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:26 0
pMDC84-ScSEIPIN
 
Resource Report
Resource Website
RRID:Addgene_96984 ScSEIPIN Saccharomyces cerevisiae Kanamycin PMID:26362606 The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html Backbone Size:12460; Vector Backbone:pMDC84; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:22:32 0
pMDC84-ScSEIPIN-Ndelta
 
Resource Report
Resource Website
RRID:Addgene_96985 ScSEIPIN Saccharomyces cerevisiae Kanamycin PMID:26362606 The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html Backbone Size:12460; Vector Backbone:pMDC84; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin N-terminal 39 nucleotides were deleted from yeast SEIPIN 2026-08-15 01:22:31 0

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